Pyrosequencing reveals the effect of mobilizing agents and lignocellulosic substrate amendment on microbial community composition in a real industrial PAH-polluted soil
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25261758
DOI
10.1016/j.jhazmat.2014.08.065
PII: S0304-3894(14)00740-7
Knihovny.cz E-zdroje
- Klíčová slova
- Creosote, HMW–PAH, Next generation sequencing (NGS), Non-ionic surfactant, Soil bioremediation, White rot fungi (WRF),
- MeSH
- Bacteria klasifikace MeSH
- biodegradace MeSH
- biodiverzita MeSH
- denaturační gradientová gelová elektroforéza MeSH
- houby klasifikace MeSH
- kreosot analýza MeSH
- látky znečišťující půdu analýza MeSH
- mezerníky ribozomální DNA genetika MeSH
- polycyklické aromatické uhlovodíky analýza MeSH
- povrchově aktivní látky chemie MeSH
- průmysl MeSH
- půda chemie MeSH
- půdní mikrobiologie * MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kreosot MeSH
- látky znečišťující půdu MeSH
- mezerníky ribozomální DNA MeSH
- polycyklické aromatické uhlovodíky MeSH
- povrchově aktivní látky MeSH
- půda MeSH
- RNA ribozomální 16S MeSH
Bacterial and fungal biodiversity throughout different biostimulation and bioaugmentation treatments applied to an industrial creosote-polluted soil were analyzed by means of polyphasic approach in order to gain insight into the microbial community structure and dynamics. Pyrosequencing data obtained from initial creosote polluted soil (after a biopiling step) revealed that Alpha and Gammaproteobacteria were the most abundant bacterial groups, whereas Fusarium and Scedosporium were the main fungal genera in the contaminated soil. At the end of 60-days laboratory scale bioremediation assays, pyrosequencing and DGGE data showed that (i) major bacterial community shifts were caused by the type of mobilizing agent added to the soil and, to a lesser extent, by the addition of lignocellulosic substrate; and (ii) the presence of the non-ionic surfactant (Brij 30) hampered the proliferation of Actinobacteria (Mycobacteriaceae) and Bacteroidetes (Chitinophagaceae) and, in the absence of lignocellulosic substrate, also impeded polycyclic aromatic hydrocarbons (PAHs) degradation. The results show the importance of implementing bioremediation experiments combined with microbiome assessment to gain insight on the effect of crucial parameters (e.g. use of additives) over the potential functions of complex microbial communities harbored in polluted soils, essential for bioremediation success.
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