Pyrosequencing reveals the effect of mobilizing agents and lignocellulosic substrate amendment on microbial community composition in a real industrial PAH-polluted soil
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25261758
DOI
10.1016/j.jhazmat.2014.08.065
PII: S0304-3894(14)00740-7
Knihovny.cz E-resources
- Keywords
- Creosote, HMW–PAH, Next generation sequencing (NGS), Non-ionic surfactant, Soil bioremediation, White rot fungi (WRF),
- MeSH
- Bacteria classification MeSH
- Biodegradation, Environmental MeSH
- Biodiversity MeSH
- Denaturing Gradient Gel Electrophoresis MeSH
- Fungi classification MeSH
- Creosote analysis MeSH
- Soil Pollutants analysis MeSH
- DNA, Ribosomal Spacer genetics MeSH
- Polycyclic Aromatic Hydrocarbons analysis MeSH
- Surface-Active Agents chemistry MeSH
- Industry MeSH
- Soil chemistry MeSH
- Soil Microbiology * MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Creosote MeSH
- Soil Pollutants MeSH
- DNA, Ribosomal Spacer MeSH
- Polycyclic Aromatic Hydrocarbons MeSH
- Surface-Active Agents MeSH
- Soil MeSH
- RNA, Ribosomal, 16S MeSH
Bacterial and fungal biodiversity throughout different biostimulation and bioaugmentation treatments applied to an industrial creosote-polluted soil were analyzed by means of polyphasic approach in order to gain insight into the microbial community structure and dynamics. Pyrosequencing data obtained from initial creosote polluted soil (after a biopiling step) revealed that Alpha and Gammaproteobacteria were the most abundant bacterial groups, whereas Fusarium and Scedosporium were the main fungal genera in the contaminated soil. At the end of 60-days laboratory scale bioremediation assays, pyrosequencing and DGGE data showed that (i) major bacterial community shifts were caused by the type of mobilizing agent added to the soil and, to a lesser extent, by the addition of lignocellulosic substrate; and (ii) the presence of the non-ionic surfactant (Brij 30) hampered the proliferation of Actinobacteria (Mycobacteriaceae) and Bacteroidetes (Chitinophagaceae) and, in the absence of lignocellulosic substrate, also impeded polycyclic aromatic hydrocarbons (PAHs) degradation. The results show the importance of implementing bioremediation experiments combined with microbiome assessment to gain insight on the effect of crucial parameters (e.g. use of additives) over the potential functions of complex microbial communities harbored in polluted soils, essential for bioremediation success.
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