Determination of lipidomic differences between human breast cancer and surrounding normal tissues using HILIC-HPLC/ESI-MS and multivariate data analysis
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Principal Component Analysis MeSH
- Chromatography, Liquid methods MeSH
- Phosphatidylcholines analysis MeSH
- Phosphatidylethanolamines analysis MeSH
- Phosphatidylinositols analysis MeSH
- Phospholipids analysis chemistry MeSH
- Spectrometry, Mass, Electrospray Ionization methods MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Humans MeSH
- Lipids analysis chemistry MeSH
- Lysophosphatidylcholines analysis MeSH
- Multivariate Analysis MeSH
- Breast Neoplasms metabolism pathology MeSH
- Reference Values MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Phosphatidylcholines MeSH
- Phosphatidylethanolamines MeSH
- Phosphatidylinositols MeSH
- Phospholipids MeSH
- Lipids MeSH
- Lysophosphatidylcholines MeSH
The comprehensive approach for the lipidomic characterization of human breast cancer and surrounding normal tissues is based on hydrophilic interaction liquid chromatography (HILIC)-electrospray ionization mass spectrometry (ESI-MS) quantitation of polar lipid classes of total lipid extracts followed by multivariate data analysis using unsupervised principal component analysis (PCA) and supervised orthogonal partial least square (OPLS). This analytical methodology is applied for the detailed lipidomic characterization of ten patients with the goal to find the statistically relevant differences between tumor and normal tissues. This strategy is selected for better visualization of differences, because the breast cancer tissue is compared with the surrounding healthy tissue of the same patient, therefore changes in the lipidome are caused predominantly by the tumor growth. A large increase of total concentrations for several lipid classes is observed, including phosphatidylinositols, phosphatidylethanolamines, phosphatidylcholines, and lysophosphatidylcholines. Concentrations of individual lipid species inside the abovementioned classes are also changed, and in some cases, these differences are statistically significant. PCA and OPLS analyses enable a clear differentiation of tumor and normal tissues based on changes of their lipidome. A notable decrease of relative abundances of ether and vinylether (plasmalogen) lipid species is detected for phosphatidylethanolamines, but no difference is apparent for phosphatidylcholines.
References provided by Crossref.org
