Different blue-aleurone wheats display major differences in chromosome composition, ranging from disomic chromosome additions, substitutions, single chromosome arm introgressions and chromosome translocation of Thinopyrum ponticum. Anthocyanins are of great importance for human health due to their antioxidant, anti-inflammatory, anti-microbial and anti-cancerogenic potential. In common wheat (Triticum aestivum L.) their content is low. However, elite lines with blue aleurone exhibit significantly increased levels of anthocyanins. These lines carry introgressed chromatin from wild relatives of wheat such as Thinopyrum ponticum and Triticum monococcum. The aim of our study was to characterize genomic constitutions of wheat lines with blue aleurone using genomic and fluorescence in situ hybridization. We used total genomic DNA of Th. ponticum and two repetitive DNA sequences (GAA repeat and the Afa family) as probes to identify individual chromosomes. This enabled precise localization of introgressed Th. ponticum chromatin. Our results revealed large variation in chromosome constitutions of the blue-aleurone wheats. Of 26 analyzed lines, 17 carried an introgression from Th. ponticum; the remaining nine lines presumably carry T. monococcum chromatin undetectable by the methods employed. Of the Th. ponticum introgressions, six different types were present, ranging from a ditelosomic addition (cv. Blue Norco) to a disomic substitution (cv. Blue Baart), substitution of complete (homologous) chromosome arms (line UC66049) and various translocations of distal parts of a chromosome arm(s). Different types of introgressions present support a hypothesis that the introgressions activate the blue aleurone trait present, but inactivated, in common wheat germplasm.

Institute of Experimental Botany Centre of the Region Haná for Biotechnological and Agricultural Research Šlechtitelů 31 78371 Olomouc Czech Republic

Zobrazit více v PubMed

Theor Appl Genet. 1991 Apr;81(4):551-8 PubMed

Ann Ist Super Sanita. 2007;43(4):369-74 PubMed

J Biomed Biotechnol. 2004;2004(5):321-325 PubMed

Carcinogenesis. 2006 May;27(5):989-96 PubMed

Genetika. 2012 Aug;48(8):926-33 PubMed

J Am Coll Nutr. 2000 Jun;19(3 Suppl):291S-299S PubMed

Free Radic Biol Med. 2000 Jul 1;29(1):51-60 PubMed

Genetics. 1996 Jun;143(2):983-99 PubMed

Chromosome Res. 2002;10(5):349-57 PubMed

Cancer Lett. 2008 Oct 8;269(2):281-90 PubMed

J Agric Food Chem. 2003 Jun 4;51(12):3560-3 PubMed

Thromb Res. 2005;116(4):327-34 PubMed

J Exp Bot. 2004 Feb;55(396):365-75 PubMed

J Invest Dermatol. 2007 Jan;127(1):222-32 PubMed

Theor Appl Genet. 1993 Apr;86(2-3):365-70 PubMed

J Agric Food Chem. 2003 Jan 1;51(1):68-75 PubMed

Theor Appl Genet. 2007 Aug;115(3):301-12 PubMed

J Agric Food Chem. 2006 Jun 28;54(13):4696-704 PubMed

PLoS One. 2013;8(2):e57994 PubMed

Genome. 1996 Dec;39(6):1062-71 PubMed

Genetics. 2005 Jun;170(2):823-9 PubMed

J Agric Food Chem. 2002 Jul 17;50(15):4183-9 PubMed

Nat Prod Rep. 2001 Jun;18(3):310-33 PubMed

Genetics. 1981 Jun;98(2):389-98 PubMed

Genome. 2006 Sep;49(9):1109-14 PubMed

J Chromatogr A. 2004 Oct 29;1054(1-2):129-41 PubMed

J Biomed Biotechnol. 2004;2004(5):306-313 PubMed

Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel