Variation in genome composition of blue-aleurone wheat
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anthocyanins MeSH
- Chromosomes, Plant genetics MeSH
- DNA, Plant genetics MeSH
- Genome, Plant * MeSH
- Genotype MeSH
- Gene Dosage MeSH
- In Situ Hybridization, Fluorescence MeSH
- Karyotype MeSH
- Triticum genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anthocyanins MeSH
- DNA, Plant MeSH
Different blue-aleurone wheats display major differences in chromosome composition, ranging from disomic chromosome additions, substitutions, single chromosome arm introgressions and chromosome translocation of Thinopyrum ponticum. Anthocyanins are of great importance for human health due to their antioxidant, anti-inflammatory, anti-microbial and anti-cancerogenic potential. In common wheat (Triticum aestivum L.) their content is low. However, elite lines with blue aleurone exhibit significantly increased levels of anthocyanins. These lines carry introgressed chromatin from wild relatives of wheat such as Thinopyrum ponticum and Triticum monococcum. The aim of our study was to characterize genomic constitutions of wheat lines with blue aleurone using genomic and fluorescence in situ hybridization. We used total genomic DNA of Th. ponticum and two repetitive DNA sequences (GAA repeat and the Afa family) as probes to identify individual chromosomes. This enabled precise localization of introgressed Th. ponticum chromatin. Our results revealed large variation in chromosome constitutions of the blue-aleurone wheats. Of 26 analyzed lines, 17 carried an introgression from Th. ponticum; the remaining nine lines presumably carry T. monococcum chromatin undetectable by the methods employed. Of the Th. ponticum introgressions, six different types were present, ranging from a ditelosomic addition (cv. Blue Norco) to a disomic substitution (cv. Blue Baart), substitution of complete (homologous) chromosome arms (line UC66049) and various translocations of distal parts of a chromosome arm(s). Different types of introgressions present support a hypothesis that the introgressions activate the blue aleurone trait present, but inactivated, in common wheat germplasm.
See more in PubMed
Theor Appl Genet. 1991 Apr;81(4):551-8 PubMed
Ann Ist Super Sanita. 2007;43(4):369-74 PubMed
J Biomed Biotechnol. 2004;2004(5):321-325 PubMed
Carcinogenesis. 2006 May;27(5):989-96 PubMed
Genetika. 2012 Aug;48(8):926-33 PubMed
J Am Coll Nutr. 2000 Jun;19(3 Suppl):291S-299S PubMed
Free Radic Biol Med. 2000 Jul 1;29(1):51-60 PubMed
Genetics. 1996 Jun;143(2):983-99 PubMed
Chromosome Res. 2002;10(5):349-57 PubMed
Cancer Lett. 2008 Oct 8;269(2):281-90 PubMed
J Agric Food Chem. 2003 Jun 4;51(12):3560-3 PubMed
Thromb Res. 2005;116(4):327-34 PubMed
J Exp Bot. 2004 Feb;55(396):365-75 PubMed
J Invest Dermatol. 2007 Jan;127(1):222-32 PubMed
Theor Appl Genet. 1993 Apr;86(2-3):365-70 PubMed
J Agric Food Chem. 2003 Jan 1;51(1):68-75 PubMed
Theor Appl Genet. 2007 Aug;115(3):301-12 PubMed
J Agric Food Chem. 2006 Jun 28;54(13):4696-704 PubMed
PLoS One. 2013;8(2):e57994 PubMed
Genome. 1996 Dec;39(6):1062-71 PubMed
Genetics. 2005 Jun;170(2):823-9 PubMed
J Agric Food Chem. 2002 Jul 17;50(15):4183-9 PubMed
Nat Prod Rep. 2001 Jun;18(3):310-33 PubMed
Genetics. 1981 Jun;98(2):389-98 PubMed
Genome. 2006 Sep;49(9):1109-14 PubMed
J Chromatogr A. 2004 Oct 29;1054(1-2):129-41 PubMed
J Biomed Biotechnol. 2004;2004(5):306-313 PubMed
