OBJECTIVE: Approximately one third of patients diagnosed with muscle-invasive urinary bladder cancer (UBC) have undetected metastases at the time of treatment of the primary tumor. Currently there are no reliable specific serum markers for monitoring and evaluating risk profiles of urothelial cancers. Several studies suggest that detection of circulating tumor cells (CTCs) may correlate with the disease status and prognosis at baseline and early in the treatment of cancers. In this study a new way of isolation and in vitro cultivation of CTCs of urinary bladder cancer was introduced. MATERIALS AND METHODS: Peripheral blood (PB) samples from 53 patients who had undergone urological procedure were evaluated using the MetaCell device (MetaCell s.r.o., Ostrava, Czech Republic). The patients enrolled in the study were both oncological patients with UBC and non-oncological patients with inflammation (14 patients). The sensitivity and quantification of CTCs were evaluated. The separated CTCs were cultured in vitro. RESULTS: 39 patients with confirmed UBC were enrolled in the study. CTCs were detected in 25 (64%) patients, and most of these patients had between 6 and 10 cells. The separated CTCs were successfully cultured in vitro. CONCLUSION: CTCs were detected in a higher percentage of patients than in other studies. This paper describes the first successful culturing of human UBC cells. The MetaCell approach used in this study enabled the capture of viable intact virgin CTCs (virgin CTC) suitable for next in vitro culturing, single cell analysis or drug testing.

Department of Laboratory Genetics University Hospital Kralovske Vinohrady Srobarova 50 100 97 Prague Czech Republic

Department of Laboratory Genetics University Hospital Kralovske Vinohrady Srobarova 50 100 97 Prague Czech Republic ; Department of Histology and Embryology Wroclaw Medical University Chalubinskiego 6a 50 368 Wroclaw Poland ; 3rd Department of Surgery 1st Faculty of Medicine Charles University Prague and University Hospital Motol 105 00 Prague Czech Republic

Department of Oncology and Division of Oncological Surgery Wroclaw Medical University ul Pasteura 1 50 367 Wrocław Poland ; Lower Silesian Oncology Centre Plac Hirszfelda 12 53 413 Wrocław Poland

Department of Pathology Masaryk's Hospital in Usti Nad Labem Krajska Zdravotni a s Socialni Pece 3316 12A40113 Usti Nad Labem Czech Republic

Department of Urology and Center of Robotic Surgery Masaryk's Hospital in Usti Nad Labem Krajska Zdravotni a s Socialni Pece 3316 12A40113 Usti Nad Labem Czech Republic

Department of Urology Regional Specialistic Hospital ul Kamienskiego 73a 51 318 Wroclaw Poland

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