Depolarization affects the lateral microdomain structure of yeast plasma membrane
Language English Country England, Great Britain Media print-electronic
Document type Editorial, Research Support, Non-U.S. Gov't
PubMed
25410771
DOI
10.1111/febs.13156
Knihovny.cz E-resources
- Keywords
- gel microdomains, lipid order, phase separation, time-resolved fluorescence, transmembrane potential,
- MeSH
- Cell Membrane metabolism MeSH
- Membrane Microdomains metabolism MeSH
- Membrane Potentials physiology MeSH
- Saccharomyces cerevisiae Proteins metabolism MeSH
- Saccharomyces cerevisiae metabolism MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Editorial MeSH
- Names of Substances
- Saccharomyces cerevisiae Proteins MeSH
We report the transmembrane voltage-induced lateral reorganization of highly-ordered lipid microdomains in the plasma membrane of living Saccharomyces cerevisiae. Using trans-parinaric acid (all-trans-9,11,13,15-octadecatetraenoic acid) as a probe of lipid order and different methods of membrane depolarization, we found that depolarization always invokes a significant reduction in the amount of gel-like microdomains in the membrane. Different depolarization mechanisms, including the application of ionophores, cell depolarization by an external electric field, depolarization by proton/hexose co-transport facilitated by HUP1 protein and a reduction of membrane potential caused by compromised respiration efficiency, yielded the same results independently of the yeast strain used. The data suggest that the voltage-induced reorganization of lateral membrane structure could play significant role in fast cellular response to acute stress conditions, as well as in other membrane microdomain-related regulatory mechanisms.
References provided by Crossref.org
Role of MCC/Eisosome in Fungal Lipid Homeostasis
There Is No Simple Model of the Plasma Membrane Organization
