Depolarization affects the lateral microdomain structure of yeast plasma membrane
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu úvodníky, práce podpořená grantem
PubMed
25410771
DOI
10.1111/febs.13156
Knihovny.cz E-zdroje
- Klíčová slova
- gel microdomains, lipid order, phase separation, time-resolved fluorescence, transmembrane potential,
- MeSH
- buněčná membrána metabolismus MeSH
- membránové mikrodomény metabolismus MeSH
- membránové potenciály fyziologie MeSH
- Saccharomyces cerevisiae - proteiny metabolismus MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- Publikační typ
- práce podpořená grantem MeSH
- úvodníky MeSH
- Názvy látek
- Saccharomyces cerevisiae - proteiny MeSH
We report the transmembrane voltage-induced lateral reorganization of highly-ordered lipid microdomains in the plasma membrane of living Saccharomyces cerevisiae. Using trans-parinaric acid (all-trans-9,11,13,15-octadecatetraenoic acid) as a probe of lipid order and different methods of membrane depolarization, we found that depolarization always invokes a significant reduction in the amount of gel-like microdomains in the membrane. Different depolarization mechanisms, including the application of ionophores, cell depolarization by an external electric field, depolarization by proton/hexose co-transport facilitated by HUP1 protein and a reduction of membrane potential caused by compromised respiration efficiency, yielded the same results independently of the yeast strain used. The data suggest that the voltage-induced reorganization of lateral membrane structure could play significant role in fast cellular response to acute stress conditions, as well as in other membrane microdomain-related regulatory mechanisms.
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