Microbial invasion and histological chorioamnionitis upregulate neutrophil-gelatinase associated lipocalin in preterm prelabor rupture of membranes
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Amniotic fluid, histological chorioamnionitis, microbial invasion of the amniotic cavity, neutrophil-gelatinase associated lipocalin, preterm prelabor rupture of membranes,
- MeSH
- biologické markery metabolismus MeSH
- chorioamnionitida metabolismus mikrobiologie MeSH
- dospělí MeSH
- kohortové studie MeSH
- lidé MeSH
- lipokalin-2 MeSH
- lipokaliny metabolismus MeSH
- mladý dospělý MeSH
- plodová voda metabolismus mikrobiologie MeSH
- předčasný odtok plodové vody metabolismus mikrobiologie MeSH
- proteiny akutní fáze metabolismus MeSH
- protoonkogenní proteiny metabolismus MeSH
- těhotenství MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biologické markery MeSH
- LCN2 protein, human MeSH Prohlížeč
- lipokalin-2 MeSH
- lipokaliny MeSH
- proteiny akutní fáze MeSH
- protoonkogenní proteiny MeSH
Our recent exploratory proteomic study suggested increased levels of neutrophil-gelatinase associated lipocalin (P80188, NGAL_HUMAN) due to microbial invasion of the amniotic cavity (MIAC) and histological chorioamnionitis (HCA) in women with preterm prelabor rupture of the membranes. In this study, we verified the proteomics findings by assessing the amniotic fluid NGAL by ELISA in the original exploratory cohort. The NGAL level was significantly higher in women positive for both MIAC and HCA compared to women with both conditions ruled out (median 75.1 ng/ml versus 27.9 ng/ml; p < 0.0001). For independent validation and to assess NGALs potential to stratify women positive for both MIAC and HCA from women in whom at least one of these conditions was absent, we subsequently designed a retrospective replication cohort. Significantly higher NGAL levels were found in women positive for both MIAC and HCA (median 65.9 ng/ml versus 34.2 ng/ml; p = 0.0061). Significantly higher levels of NGAL were confirmed only in strata below 32 weeks of gestation. Based on the observed likelihood ratio, the best predictive cutoff level (47.1 ng/ml) was evaluated in both cohorts. Data from the verification cohort implied that NGAL is a valuable clinical marker for revealing MIAC leading to HCA; however, this potential was not replicated in the replication cohort.
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