Preterm prelabour rupture of membranes beyond the 34th week of gestation (late PPROM) is frequently associated with the risk of the microbial invasion of the amniotic fluid (MIAC) and histological chorioamnionitis (HCA). Hence, we employed a Tandem Mass Tag-based approach to uncover amniotic fluid proteome response to the presence of MIAC and HCA in late PPROM. Protein dysregulation was associated with only five cases in the group of 15 women with confirmed MIAC and HCA. Altogether, 138 amniotic fluid proteins were changed in these five cases exclusively. These proteins were particularly associated with excessive neutrophil responses to infection, such as neutrophil degranulation and extracellular trap formation. We believe that the quantification of these proteins in amniotic fluid may assist in revealing women with the highest risk of excessive inflammatory response in late PPROM.

BIOCEV 1st Faculty of Medicine Charles University Prague Czech Republic

Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic

Department of Analytical Chemistry Faculty of Pharmacy in Hradec Kralove Charles University Hradec Kralove Czech Republic

Department of Biological and Biochemical Sciences Faculty of Chemical Technology University of Pardubice Pardubice Czech Republic

Department of Genetics and Bioinformatics Domain of Health Data and Digitalization Institute of Public Health Oslo Norway

Department of Metabolomics Institute of Physiology Czech Academy of Sciences Prague Czech Republic

Department of Obstetrics and Gynecology Institute of Clinical Science Sahlgrenska Academy University of Gothenburg Gothenburg Sweden

Department of Obstetrics and Gynecology University Hospital Hradec Kralove Charles University Faculty of Medicine in Hradec Kralove Hradec Kralove Czech Republic

Fingerland's Department of Pathology University Hospital in Hradec Kralove Charles University Faculty of Medicine Hradec Kralove Hradec Kralove Czech Republic

Food and Nutrition Science Department of Biology and Biological Engineering Chalmers University of Technology Gothenburg Sweden

Institute of Clinical Biochemistry and Diagnostics University Hospital Hradec Kralove Hradec Kralove Czech Republic

Institute of Clinical Immunology and Allergology University Hospital Hradec Kralove Hradec Kralove Czech Republic

Institute of Clinical Microbiology University Hospital Hradec Kralove Hradec Kralove Czech Republic

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