Seasonal changes in the composition of storage and membrane lipids in overwintering larvae of the codling moth, Cydia pomonella
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25436961
DOI
10.1016/j.jtherbio.2014.08.011
PII: S0306-4565(14)00128-4
Knihovny.cz E-resources
- Keywords
- Cold tolerance, Fatty acids, Homeoviscous adaptation, Membrane fluidity, Phospholipids, Triacylglycerols,
- MeSH
- Membrane Fluidity MeSH
- Linoleic Acid metabolism MeSH
- Larva metabolism MeSH
- Membrane Lipids metabolism MeSH
- Moths MeSH
- Palmitates metabolism MeSH
- Cold-Shock Response MeSH
- Seasons * MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Linoleic Acid MeSH
- Membrane Lipids MeSH
- Palmitates MeSH
The codling moth (Cydia pomonella) is a major insect pest of apples worldwide. It overwinters as a diapausing fifth instar larva. The overwintering is often a critical part of the insect life-cycle in temperate zone. This study brings detailed analysis of seasonal changes in lipid composition and fluidity in overwintering larvae sampled in the field. Fatty acid composition of triacylglycerol (TG) depots in the fat body and relative proportions of phospholipid (PL) molecular species in biological membranes were analyzed. In addition, temperature of melting (Tm) in TG depots was assessed by using differential scanning calorimetry and the conformational order (fluidity) of PL membranes was analyzed by measuring the anisotropy of fluorescence polarization of diphenylhexatriene probe in membrane vesicles. We observed a significant increase of relative proportion of linoleic acid (C18:2n6) at the expense of palmitic acid (C16:0) in TG depots during the larval transition to diapause accompanied with decreasing melting temperature of total lipids, which might increase the accessibility of depot fats for enzymatic breakdown during overwintering. The fluidity of membranes was maintained very high irrespective of developmental mode or seasonally changing acclimation status of larvae. The seasonal changes in PL composition were relatively small. We discuss these results in light of alternative survival strategies of codling moth larvae (supercooling vs. freezing), variability and low predictability of environmental conditions, and other cold tolerance mechanisms such as extending the supercooling capacity and massive accumulation of cryoprotective metabolites.
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