Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Review
PubMed
25458555
DOI
10.1016/j.micres.2014.09.006
PII: S0944-5013(14)00115-3
Knihovny.cz E-resources
- Keywords
- Bacterial secretion, Gram-negative bacteria, Host–pathogen interaction, Outer membrane vesicles, Vaccine development,
- MeSH
- Cell Wall * immunology metabolism MeSH
- Cell Fractionation methods MeSH
- Stress, Physiological MeSH
- Genetic Engineering MeSH
- Gram-Negative Bacteria * genetics immunology metabolism MeSH
- Secretory Vesicles * immunology metabolism MeSH
- Subcellular Fractions MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Outer membrane vesicles secreted by gram-negative bacteria play an important role in bacterial physiology as well as in virulence and host-pathogen interaction. Isolated vesicles of some bacteria have also been studied for their immunomodulatory potential in the vaccine development. However, the production of vesicles in sufficient amount, purity and reproducibility remains a critical challenge for subsequent analyses in most bacteria. In the present review methods of production, isolation, purification and quantification of outer membrane vesicles are summarized and discussed.
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