Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, Research Support, U.S. Gov't, Non-P.H.S., přehledy
PubMed
25458555
DOI
10.1016/j.micres.2014.09.006
PII: S0944-5013(14)00115-3
Knihovny.cz E-zdroje
- Klíčová slova
- Bacterial secretion, Gram-negative bacteria, Host–pathogen interaction, Outer membrane vesicles, Vaccine development,
- MeSH
- buněčná stěna * imunologie metabolismus MeSH
- frakcionace buněk metody MeSH
- fyziologický stres MeSH
- genetické inženýrství MeSH
- gramnegativní bakterie * genetika imunologie metabolismus MeSH
- sekreční vezikuly * imunologie metabolismus MeSH
- subcelulární frakce MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Outer membrane vesicles secreted by gram-negative bacteria play an important role in bacterial physiology as well as in virulence and host-pathogen interaction. Isolated vesicles of some bacteria have also been studied for their immunomodulatory potential in the vaccine development. However, the production of vesicles in sufficient amount, purity and reproducibility remains a critical challenge for subsequent analyses in most bacteria. In the present review methods of production, isolation, purification and quantification of outer membrane vesicles are summarized and discussed.
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