Elevated levels of 14-3-3 proteins, serotonin, gamma enolase and pyruvate kinase identified in clinical samples from patients diagnosed with colorectal cancer
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25540887
DOI
10.1016/j.cca.2014.12.005
PII: S0009-8981(14)00539-7
Knihovny.cz E-zdroje
- Klíčová slova
- 14-3-3 Proteins, Biomarkers, Colorectal cancer, Mass spectrometry, Proteomics, Pyruvate kinase,
- MeSH
- fosfopyruváthydratasa krev metabolismus MeSH
- hmotnostní spektrometrie MeSH
- kolorektální nádory krev diagnóza MeSH
- lidé středního věku MeSH
- lidé MeSH
- metabolomika MeSH
- proteiny 14-3-3 krev metabolismus MeSH
- proteomika MeSH
- pyruvátkinasa krev metabolismus MeSH
- senioři MeSH
- serotonin krev metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fosfopyruváthydratasa MeSH
- proteiny 14-3-3 MeSH
- pyruvátkinasa MeSH
- serotonin MeSH
BACKGROUND: Colorectal cancer (CRC), a heterogeneous disease that is common in both men and women, continues to be one of the predominant cancers worldwide. Lifestyle, diet, environmental factors and gene defects all contribute towards CRC development risk. Therefore, the identification of novel biomarkers to aid in the management of CRC is crucial. The aim of the present study was to identify candidate biomarkers for CRC, and to develop a better understanding of their role in tumourogenesis. METHODS: In this study, both plasma and tissue samples from patients diagnosed with CRC, together with non-malignant and normal controls were examined using mass spectrometry based proteomics and metabolomics approaches. RESULTS: It was established that the level of several biomolecules, including serotonin, gamma enolase, pyruvate kinase and members of the 14-3-3 family of proteins, showed statistically significant changes when comparing malignant versus non-malignant patient samples, with a distinct pattern emerging mirroring cancer cell energy production. CONCLUSION: The diagnosis and management of CRC could be enhanced by the discovery and validation of new candidate biomarkers, as found in this study, aimed at facilitating early detection and/or patient stratification together with providing information on the complex behaviour of cancer cells.
1st Medical Faculty of Charles University and Thomayer University Hospital Prague Czech Republic
Department of Colorectal Surgery AMNCH Hospital Dublin 24 Ireland
Human Genetics Foundation Turin Italy
National Institute for Cellular Biotechnology Dublin City University Glasnevin Dublin 9 Ireland
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