Elevated levels of 14-3-3 proteins, serotonin, gamma enolase and pyruvate kinase identified in clinical samples from patients diagnosed with colorectal cancer
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25540887
DOI
10.1016/j.cca.2014.12.005
PII: S0009-8981(14)00539-7
Knihovny.cz E-resources
- Keywords
- 14-3-3 Proteins, Biomarkers, Colorectal cancer, Mass spectrometry, Proteomics, Pyruvate kinase,
- MeSH
- Phosphopyruvate Hydratase blood metabolism MeSH
- Mass Spectrometry MeSH
- Colorectal Neoplasms blood diagnosis MeSH
- Middle Aged MeSH
- Humans MeSH
- Metabolomics MeSH
- 14-3-3 Proteins blood metabolism MeSH
- Proteomics MeSH
- Pyruvate Kinase blood metabolism MeSH
- Aged MeSH
- Serotonin blood metabolism MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Phosphopyruvate Hydratase MeSH
- 14-3-3 Proteins MeSH
- Pyruvate Kinase MeSH
- Serotonin MeSH
BACKGROUND: Colorectal cancer (CRC), a heterogeneous disease that is common in both men and women, continues to be one of the predominant cancers worldwide. Lifestyle, diet, environmental factors and gene defects all contribute towards CRC development risk. Therefore, the identification of novel biomarkers to aid in the management of CRC is crucial. The aim of the present study was to identify candidate biomarkers for CRC, and to develop a better understanding of their role in tumourogenesis. METHODS: In this study, both plasma and tissue samples from patients diagnosed with CRC, together with non-malignant and normal controls were examined using mass spectrometry based proteomics and metabolomics approaches. RESULTS: It was established that the level of several biomolecules, including serotonin, gamma enolase, pyruvate kinase and members of the 14-3-3 family of proteins, showed statistically significant changes when comparing malignant versus non-malignant patient samples, with a distinct pattern emerging mirroring cancer cell energy production. CONCLUSION: The diagnosis and management of CRC could be enhanced by the discovery and validation of new candidate biomarkers, as found in this study, aimed at facilitating early detection and/or patient stratification together with providing information on the complex behaviour of cancer cells.
1st Medical Faculty of Charles University and Thomayer University Hospital Prague Czech Republic
Department of Colorectal Surgery AMNCH Hospital Dublin 24 Ireland
Human Genetics Foundation Turin Italy
National Institute for Cellular Biotechnology Dublin City University Glasnevin Dublin 9 Ireland
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