Eggs of sterlet are discharged outside into ambient aquatic environment where egg activation and fertilization occur. Effects of different activation media including freshwater and clay suspension on protein abundances of egg were quantified in sterlet Acipenser ruthenus. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification in the eggs of five females. No significant (p > 0.05) difference was found between protein abundances in eggs activated with different media. However, results showed significant (p < 0.05, fold change ≥2) reduction in the abundances of nine proteins including six glycoproteins, enolase and heat shock protein in activated groups compared to freshly ovulated eggs as control. The fact that abundance of proteasome subunit alpha significantly reduced only in eggs which were activated by clay suspension suggests that activation medium can somehow intervene with protein regulation during fertilization. In conclusion, external fertilization in sturgeon egg is accompanied by huge release of proteins into the external environment that may participate in the construction of a transient microenvironment around egg for attraction and protection of spermatozoa to ensure ensuing fertilization. Data are available via ProteomeXchange with identifier PXD006232.

• MeSH
• fertilizace * MeSH
• fosfopyruváthydratasa metabolismus   MeSH
• glykoproteiny metabolismus   MeSH
• ovum metabolismus   MeSH
• proteiny tepelného šoku metabolismus   MeSH
• proteom metabolismus   MeSH
• ryby metabolismus   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND: Colorectal cancer (CRC), a heterogeneous disease that is common in both men and women, continues to be one of the predominant cancers worldwide. Lifestyle, diet, environmental factors and gene defects all contribute towards CRC development risk. Therefore, the identification of novel biomarkers to aid in the management of CRC is crucial. The aim of the present study was to identify candidate biomarkers for CRC, and to develop a better understanding of their role in tumourogenesis. METHODS: In this study, both plasma and tissue samples from patients diagnosed with CRC, together with non-malignant and normal controls were examined using mass spectrometry based proteomics and metabolomics approaches. RESULTS: It was established that the level of several biomolecules, including serotonin, gamma enolase, pyruvate kinase and members of the 14-3-3 family of proteins, showed statistically significant changes when comparing malignant versus non-malignant patient samples, with a distinct pattern emerging mirroring cancer cell energy production. CONCLUSION: The diagnosis and management of CRC could be enhanced by the discovery and validation of new candidate biomarkers, as found in this study, aimed at facilitating early detection and/or patient stratification together with providing information on the complex behaviour of cancer cells.

• MeSH
• fosfopyruváthydratasa krev   metabolismus   MeSH
• hmotnostní spektrometrie MeSH
• kolorektální nádory krev   diagnóza   MeSH
• lidé středního věku MeSH
• lidé MeSH
• metabolomika MeSH
• proteiny 14-3-3 krev   metabolismus   MeSH
• proteomika MeSH
• pyruvátkinasa krev   metabolismus   MeSH
• senioři MeSH
• serotonin krev   metabolismus   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The auxiliary alpha2delta-1 subunit of voltage-gated calcium channels is up-regulated in dorsal root ganglion neurons following peripheral somatosensory nerve damage, in several animal models of neuropathic pain. The alpha2delta-1 protein has a mainly presynaptic localization, where it is associated with the calcium channels involved in neurotransmitter release. Relevant to the present study, alpha2delta-1 has been shown to be the therapeutic target of the gabapentinoid drugs in their alleviation of neuropathic pain. These drugs are also used in the treatment of certain epilepsies. In this study we therefore examined whether the level or distribution of alpha2delta-1 was altered in the hippocampus following experimental induction of epileptic seizures in rats, using both the kainic acid model of human temporal lobe epilepsy, in which status epilepticus is induced, and the tetanus toxin model in which status epilepticus is not involved. The main finding of this study is that we did not identify somatic overexpression of alpha2delta-1 in hippocampal neurons in either of the epilepsy models, unlike the upregulation of alpha2delta-1 that occurs following peripheral nerve damage to both somatosensory and motor neurons. However, we did observe local reorganization of alpha2delta-1 immunostaining in the hippocampus only in the kainic acid model, where it was associated with areas of neuronal cell loss, as indicated by absence of NeuN immunostaining, dendritic loss, as identified by areas where microtubule-associated protein-2 immunostaining was missing, and reactive gliosis, determined by regions of strong OX42 staining. Crown Copyright © 2014. Published by Elsevier Ltd. All rights reserved.

• MeSH
• antigeny CD11b metabolismus   MeSH
• buněčná smrt MeSH
• epilepsie chemicky indukované   komplikace   patologie   MeSH
• fosfopyruváthydratasa metabolismus   MeSH
• glióza etiologie   MeSH
• hipokampus * metabolismus   MeSH
• krysa rodu rattus MeSH
• kyselina kainová toxicita   MeSH
• ligace škodlivé účinky   MeSH
• modely nemocí na zvířatech MeSH
• neuralgie etiologie   komplikace   patologie   MeSH
• neurotoxiny toxicita   MeSH
• potkani Sprague-Dawley MeSH
• proteiny asociované s mikrotubuly metabolismus   MeSH
• regulace genové exprese fyziologie   účinky léků   MeSH
• spinální ganglia * metabolismus   MeSH
• tetanový toxin toxicita   MeSH
• vápníkové kanály - typ L MeSH
• vápníkové kanály * metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH

• MeSH
• antigen CA-125 metabolismus   MeSH
• antigeny nádorové metabolismus   MeSH
• ascitická tekutina MeSH
• fosfopyruváthydratasa diagnostické užití   metabolismus   MeSH
• imunoradiometrická analýza MeSH
• karcinoembryonální antigen analýza   krev   MeSH
• keratiny MeSH
• lidé MeSH
• molekuly buněčné adheze metabolismus   MeSH
• monoklonální protilátky MeSH
• mucin 1 krev   MeSH
• nádorové biomarkery * klasifikace   MeSH
• nádory plic * diagnóza   MeSH
• nemoci jater MeSH
• nemoci ledvin MeSH
• peptid uvolňující gastrin metabolismus   MeSH
• skvamocelulární nádory MeSH
• Check Tag
• lidé MeSH

Pseudomonas aeruginosa is one of the pathogenic bacteria which utilize binding of the host plasminogen (Plg) to promote their invasion throughout the host tissues. In the present study, we confirmed that P. aeruginosa exhibits binding affinity for human plasminogen. Furthermore, we showed that the protein detected on the cell wall of P. aeruginosa and binding human plasminogen is an enolase-like protein. The hypothesis that alpha-enolase, a cytoplasmatic glycolytic enzyme, resides also on the cell surface of the bacterium was supported by electron microscopy analysis. The plasminogen-binding activity of bacterial cell wall outer membrane enolase-like protein was examined by immunoblotting assay.

• MeSH
• fosfopyruváthydratasa metabolismus   MeSH
• imunoblotting MeSH
• imunoelektronová mikroskopie MeSH
• lidé MeSH
• plazminogen metabolismus   MeSH
• proteiny vnější bakteriální membrány metabolismus   MeSH
• Pseudomonas aeruginosa enzymologie   metabolismus   MeSH
• vazba proteinů MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND/AIM: Tumor biomarkers are used for diagnostics and follow-up monitoring of patients with non-small cell lung cancer (NSCLC). We focused on the predictive role of neuron-specific enolase (NSE) and thymidine [corrected] kinase (TK) in patients with advanced-stage NSCLC treated with epidermal growth factor tyrosine kinase inhibitors (EGFR-TKIs). PATIENTS AND METHODS: In a total of 163 patients with advanced-stage (IIIB or IV) NSCLC treated with EGFR-TKIs (erlotinib or gefitinib), pre-treatment levels of NSE and TK were measured. RESULTS: We observed significantly shorter progression-free (PFS) and overall survival (OS) in patients with high NSE levels (p=0.002; p=0.003) and also in those with high TK levels (p=0.026; p=0.020). The multivariate Cox proportional hazards model confirmed that high NSE is a strong independent predictive factor for short PFS (hazard ratio; HR=2.36; p=0.003). CONCLUSION: High pre-treatment serum levels of NSE is an independent biomarker predicting poor outcome of patients with NSCLC treated with EGFR-TKIs.

• MeSH
• antitumorózní látky terapeutické užití   MeSH
• dospělí MeSH
• erbB receptory antagonisté a inhibitory   MeSH
• fosfopyruváthydratasa metabolismus   MeSH
• inhibitory proteinkinas terapeutické užití   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádory plic farmakoterapie   enzymologie   mortalita   patologie   MeSH
• nemalobuněčný karcinom plic farmakoterapie   enzymologie   mortalita   patologie   MeSH
• prognóza MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• staging nádorů MeSH
• thymidinkináza metabolismus   MeSH
• výsledek terapie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Sturgeon and paddlefish (Acipenseriformes), the source of roe consumed as caviar, are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish: Siberian sturgeon (Acipenser baerii), sterlet (A. ruthenus), Russian sturgeon (A. gueldenstaedtii), starry sturgeon (A. stellatus), beluga (Huso huso), and Mississippi paddlefish (Polyodon spathula). Protein profiles of spermatozoa were determined by isoelectric focusing and two-dimensional electrophoresis (2-DE) high-resolution gels. The peptides, previously selected by 2-DE analysis as potentially species-specific, were obtained by "in-gel" tryptic digestion, followed by matrix-associated laser desorption/ionization time-of-flight/mass spectrometry (MALDI-TOF/MS). Among the 23 protein spots selected, 14 were identified as isoforms of enolase B present in all species, but with different isoelectric points or molecular mass. Exceptions were A. ruthenus and H. huso, species with a close phylogenetic relationship. Glycerol-3-phosphate dehydrogenase was detected exclusively in P. spathula. Phosphoglycerate kinase was detected only in A. ruthenus and H. huso, and 3 additional proteins (fructose bisphosphate aldolase A-2, glycogen phosphorylase type IV and glyceraldehyde-3-phosphate dehydrogenase) were found exclusively in A. gueldenstaedtii and H. huso. This study points to the application of proteomics for differential characterization and comparative studies of acipenseriform species at the molecular level.

• MeSH
• 2D gelová elektroforéza metody   MeSH
• aldolasa genetika   metabolismus   MeSH
• biologické markery metabolismus   MeSH
• druhová specificita MeSH
• fosfoglycerátkinasa genetika   metabolismus   MeSH
• fosfopyruváthydratasa genetika   metabolismus   MeSH
• glycerolfosfátdehydrogenasa genetika   metabolismus   MeSH
• isoelektrická fokusace metody   MeSH
• izoenzymy genetika   metabolismus   MeSH
• peptidové mapování metody   MeSH
• peptidy genetika   metabolismus   MeSH
• proteomika metody   MeSH
• ryby klasifikace   genetika   metabolismus   MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice metody   MeSH
• spermie metabolismus   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Geografické názvy
• Mississippi MeSH
• Sibiř MeSH

OBJECTIVES: The aim of this study was to evaluate the correlation of neuron specific enolase (NSE), protein S100B and time-profile of Glasgow Coma Score (GCS) development with metallothionein (MT) blood levels in patients with traumatic brain injury (TBI) during 10 days of hospitalization. Patients were divided into 2 groups with respect to NSE and S100B levels - with (group I) and without (group II) GCS improvement. METHODS: Serum NSE and S100B concentrations were measured by immunochemical methods; serum metallothionein concentration by electrochemical technique. Cortical biopsies were investigated immunohistochemically and by electron microscope. A cDNA microarray containing 700 gene probes was used to study the changes in gene expression in the ipsilateral cortex. RESULTS: Values of MT in the blood of group I showed a non-significant decrease compared to group II during 1-3 days after admission. There was an increase of MT during 4-8 days in comparison with values of 1-3 days. The highest value of MT during hospitalization was found in a patient with diffuse axonal injury (group II). The data of cDNA microarray suggested an increase in expression of gene transcripts for oxygen free radical scavenger proteins corresponding with the increase of MT during 4-8 days in both groups. CONCLUSIONS: The experimental data indicate that monitoring the content of MT in patients with trauma brain injury would be a suitable approach to evaluate the degree of injury or duration of prolonging unconsciousness, particularly in diagnosis of diffuse axonal injury.

• MeSH
• fosfopyruváthydratasa * krev   metabolismus   MeSH
• lidé MeSH
• magnetická rezonanční tomografie MeSH
• metalothionein * krev   MeSH
• neurotrofní faktory * krev   metabolismus   MeSH
• poranění mozku * krev   metabolismus   radiografie   MeSH
• proteiny S100 * krev   metabolismus   MeSH
• stanovení celkové genové exprese MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH

AIMS: Spermatocytic seminoma is a rare germ cell derived tumour of the testis that occurs mainly in older men. We analysed the expression of recently discovered markers for germ cell differentiation and the mitosis-meiosis transition in order to define the antigen profile for diagnostic purposes and to clarify the biology and histogenesis of spermatocytic seminoma. METHODS AND RESULTS: Twenty-five spermatocytic seminomas were examined for immunohistochemical expression of germ cell-specific onco-fetal antigens and proteins involved in regulation of germ cell division, DNA repair and differentiation. The panel included Chk2, p19INK4d, p53, MAGE-A4, KIT, TRA-1-60, neurone-specific enolase and placental-like alkaline phosphatase. Four of these proteins/antigens have never before been investigated in spermatocytic seminoma. Proteins highly expressed in gonocytes and spermatogonia, such as Chk2, MAGE-A4 and neurone-specific enolase, were consistently present in spermatocytic seminoma. Antigens expressed in embryonic germ cells but not in the normal adult testis, e.g. TRA-1-60, were undetectable, with the exception of p53 protein, which was demonstrated in 80% of cases. A proto-oncogene p19INK4d, which is involved in the transition from mitotic to meiotic division in germ cells, was not detected in spermatocytic seminoma. CONCLUSIONS: The investigation provided new information concerning the expression of Chk2, MAGE-A4, neurone-specific enolase and p19INK4d in spermatocytic seminoma. The pattern of expression is highly consistent with the origin of spermatocytic seminoma from a premeiotic germ cell, which has lost embryonic traits and has committed to spermatogenic lineage but has not yet passed the meiotic checkpoint, most probably from the spermatogonium of the adult testis.

• MeSH
• antigeny nádorové metabolismus   MeSH
• checkpoint kinasa 2 MeSH
• dospělí MeSH
• fosfopyruváthydratasa metabolismus   MeSH
• imunoenzymatické techniky MeSH
• inhibitor p16 cyklin-dependentní kinasy metabolismus   MeSH
• inhibitor p19 cyklin-dependentní kinasy MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádorové biomarkery * metabolismus   MeSH
• nádorové proteiny * metabolismus   MeSH
• nádorový supresorový protein p53 metabolismus   MeSH
• protein-serin-threoninkinasy * MeSH
• proteinkinasy metabolismus   MeSH
• proteiny buněčného cyklu * MeSH
• seminom etiologie   metabolismus   patologie   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• testikulární nádory etiologie   metabolismus   patologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH

• MeSH
• EDTA krev   metabolismus   MeSH
• erytrocyty * cytologie   metabolismus   účinky léků   MeSH
• fosfopyruváthydratasa krev   metabolismus   účinky léků   MeSH
• hematologické testy * metody   využití   MeSH
• krev - fyziologické jevy účinky léků   MeSH
• kyselina mléčná izolace a purifikace   krev   metabolismus   MeSH
• kyselina pyrohroznová * krev   metabolismus   MeSH
• laktáty izolace a purifikace   krev   metabolismus   MeSH
• lidé MeSH
• oxaláty izolace a purifikace   krev   metabolismus   MeSH
• spektrofotometrie metody   využití   MeSH
• statistika jako téma MeSH
• Check Tag
• lidé MeSH