The auxiliary alpha2delta-1 subunit of voltage-gated calcium channels is up-regulated in dorsal root ganglion neurons following peripheral somatosensory nerve damage, in several animal models of neuropathic pain. The alpha2delta-1 protein has a mainly presynaptic localization, where it is associated with the calcium channels involved in neurotransmitter release. Relevant to the present study, alpha2delta-1 has been shown to be the therapeutic target of the gabapentinoid drugs in their alleviation of neuropathic pain. These drugs are also used in the treatment of certain epilepsies. In this study we therefore examined whether the level or distribution of alpha2delta-1 was altered in the hippocampus following experimental induction of epileptic seizures in rats, using both the kainic acid model of human temporal lobe epilepsy, in which status epilepticus is induced, and the tetanus toxin model in which status epilepticus is not involved. The main finding of this study is that we did not identify somatic overexpression of alpha2delta-1 in hippocampal neurons in either of the epilepsy models, unlike the upregulation of alpha2delta-1 that occurs following peripheral nerve damage to both somatosensory and motor neurons. However, we did observe local reorganization of alpha2delta-1 immunostaining in the hippocampus only in the kainic acid model, where it was associated with areas of neuronal cell loss, as indicated by absence of NeuN immunostaining, dendritic loss, as identified by areas where microtubule-associated protein-2 immunostaining was missing, and reactive gliosis, determined by regions of strong OX42 staining.
- MeSH
- antigeny CD11b metabolismus MeSH
- buněčná smrt MeSH
- epilepsie chemicky indukované komplikace patologie MeSH
- fosfopyruváthydratasa metabolismus MeSH
- glióza etiologie MeSH
- hipokampus * metabolismus MeSH
- krysa rodu rattus MeSH
- kyselina kainová toxicita MeSH
- ligace škodlivé účinky MeSH
- modely nemocí na zvířatech MeSH
- neuralgie etiologie komplikace patologie MeSH
- neurotoxiny toxicita MeSH
- potkani Sprague-Dawley MeSH
- proteiny asociované s mikrotubuly metabolismus MeSH
- regulace genové exprese fyziologie účinky léků MeSH
- spinální ganglia * metabolismus MeSH
- tetanový toxin toxicita MeSH
- vápníkové kanály - typ L MeSH
- vápníkové kanály * metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
