Temperature dependence of production of structured triacylglycerols in the alga Trachydiscus minutus
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25564261
DOI
10.1016/j.phytochem.2014.12.013
PII: S0031-9422(14)00545-7
Knihovny.cz E-resources
- Keywords
- Alga, Chiral-LC/APCI-MS, Eustigmatophyceae, Structured triacylglycerols, Temperature, Trachydiscus minutus,
- MeSH
- Stramenopiles chemistry MeSH
- Fatty Acids, Unsaturated analysis MeSH
- Stereoisomerism MeSH
- Temperature MeSH
- Triglycerides chemistry isolation & purification metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fatty Acids, Unsaturated MeSH
- Triglycerides MeSH
This study describes the identification of regioisomers and enantiomers of triacylglycerols of C20 polyunsaturated fatty acids (PUFAs) in the alga Trachydiscus minutus cultivated at different temperatures using reversed- and chiral-phase liquid chromatography-mass spectrometry. The use of the two different phases contributes to ready identification, both qualitative and semiquantitative, of regioisomers and enantiomers of triacylglycerols containing eicosapentaenoic and arachidonic in the molecule. The ratio of regioisomers and enantiomers of triacylglycerols (TAG) depends on the temperature of cultivation; with lowering temperature the proportion of the achiral TAG increases and the enantiomer ratio diverges from 1:1.
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