Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25588196
DOI
10.1089/mdr.2014.0210
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- beta-Lactamases genetics metabolism MeSH
- beta-Lactam Resistance genetics MeSH
- Enterobacteriaceae drug effects genetics isolation & purification MeSH
- Enterobacteriaceae Infections diagnosis drug therapy microbiology MeSH
- Gene Expression MeSH
- Respiratory Tract Infections diagnosis drug therapy microbiology MeSH
- Real-Time Polymerase Chain Reaction methods MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Plasmids chemistry metabolism MeSH
- Retrospective Studies MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- beta-Lactamases MeSH
The infections caused by extended-spectrum β-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients' clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using real-time PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment.
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