Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cell Respiration drug effects MeSH
- Humans MeSH
- Mitochondria drug effects metabolism MeSH
- Cell Line, Tumor MeSH
- Oxidation-Reduction drug effects MeSH
- Antineoplastic Agents pharmacology MeSH
- Reactive Oxygen Species metabolism MeSH
- Oxygen Consumption drug effects MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antineoplastic Agents MeSH
- Reactive Oxygen Species MeSH
Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, where they may suppress mitochondrial respiration. It is therefore important to evaluate this process and understand how this is modulated by small molecules. Here, we describe the general methodology to assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted to mitochondria on this process, and also how to assess this in tumor tissue.
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