Fluorescent magnetic nanoparticles for cell labeling: flux synthesis of manganite particles and novel functionalization of silica shell
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25702866
DOI
10.1016/j.jcis.2015.01.071
PII: S0021-9797(15)00117-4
Knihovny.cz E-zdroje
- Klíčová slova
- Cell labeling, Dual probes, MRI, Magnetic nanoparticles, Manganites, Molten salt synthesis, Silica coating,
- MeSH
- fibroblasty cytologie metabolismus MeSH
- fluorescence MeSH
- fluorescenční protilátková technika MeSH
- HeLa buňky MeSH
- Jurkat buňky MeSH
- kultivované buňky MeSH
- kůže cytologie metabolismus MeSH
- lanthan chemie MeSH
- lidé MeSH
- magnetická rezonanční tomografie MeSH
- magnetické nanočástice chemie MeSH
- membránové glykoproteiny asociované s lyzozomy imunologie metabolismus MeSH
- monoklonální protilátky imunologie MeSH
- oxid křemičitý chemie MeSH
- povrchové vlastnosti MeSH
- průtoková cytometrie MeSH
- silany chemie MeSH
- sloučeniny manganu chemie MeSH
- stroncium chemie MeSH
- transmisní elektronová mikroskopie MeSH
- velikost částic MeSH
- viabilita buněk MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- LAMP1 protein, human MeSH Prohlížeč
- lanthan MeSH
- magnetické nanočástice MeSH
- manganite MeSH Prohlížeč
- membránové glykoproteiny asociované s lyzozomy MeSH
- monoklonální protilátky MeSH
- oxid křemičitý MeSH
- silany MeSH
- sloučeniny manganu MeSH
- stroncium MeSH
- tetraethoxysilane MeSH Prohlížeč
Novel synthetic approaches for the development of multimodal imaging agents with high chemical stability are demonstrated. The magnetic cores are based on La0.63Sr0.37MnO3 manganite prepared as individual grains using a flux method followed by additional thermal treatment in a protective silica shell allowing to enhance their magnetic properties. The cores are then isolated and covered de novo with a hybrid silica layer formed through the hydrolysis and polycondensation of tetraethoxysilane and a fluorescent silane synthesized from rhodamine, piperazine spacer, and 3-iodopropyltrimethoxysilane. The aminoalkyltrialkoxysilanes are strictly avoided and the resulting particles are hydrolytically stable and do not release dye. The high colloidal stability of the material and the long durability of the fluorescence are reinforced by an additional silica layer on the surface of the particles. Structural and magnetic studies of the products using XRD, TEM, and SQUID magnetometry confirm the importance of the thermal treatment and demonstrate that no mechanical treatment is required for the flux-synthesized manganite. Detailed cell viability tests show negligible or very low toxicity at concentrations at which excellent labeling is achieved. Predominant localization of nanoparticles in lysosomes is confirmed by immunofluorescence staining. Relaxometric and biological studies suggest that the functionalized nanoparticles are suitable for imaging applications.
Institute of Clinical and Experimental Medicine Vídeňská 1958 140 21 Praha 4 Czech Republic
Institute of Physics AS CR Cukrovarnická 10 162 00 Praha 6 Czech Republic
Laboratoire de Chimie UMR 5182 ENS CNRS UCBL 46 allée d'Italie 69364 Lyon cedex 07 France
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