Fluorescent magnetic nanoparticles for cell labeling: flux synthesis of manganite particles and novel functionalization of silica shell
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25702866
DOI
10.1016/j.jcis.2015.01.071
PII: S0021-9797(15)00117-4
Knihovny.cz E-resources
- Keywords
- Cell labeling, Dual probes, MRI, Magnetic nanoparticles, Manganites, Molten salt synthesis, Silica coating,
- MeSH
- Fibroblasts cytology metabolism MeSH
- Fluorescence MeSH
- Fluorescent Antibody Technique MeSH
- HeLa Cells MeSH
- Jurkat Cells MeSH
- Cells, Cultured MeSH
- Skin cytology metabolism MeSH
- Lanthanum chemistry MeSH
- Humans MeSH
- Magnetic Resonance Imaging MeSH
- Magnetite Nanoparticles chemistry MeSH
- Lysosomal Membrane Proteins immunology metabolism MeSH
- Antibodies, Monoclonal immunology MeSH
- Silicon Dioxide chemistry MeSH
- Surface Properties MeSH
- Flow Cytometry MeSH
- Silanes chemistry MeSH
- Manganese Compounds chemistry MeSH
- Strontium chemistry MeSH
- Microscopy, Electron, Transmission MeSH
- Particle Size MeSH
- Cell Survival MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- LAMP1 protein, human MeSH Browser
- Lanthanum MeSH
- Magnetite Nanoparticles MeSH
- manganite MeSH Browser
- Lysosomal Membrane Proteins MeSH
- Antibodies, Monoclonal MeSH
- Silicon Dioxide MeSH
- Silanes MeSH
- Manganese Compounds MeSH
- Strontium MeSH
- tetraethoxysilane MeSH Browser
Novel synthetic approaches for the development of multimodal imaging agents with high chemical stability are demonstrated. The magnetic cores are based on La0.63Sr0.37MnO3 manganite prepared as individual grains using a flux method followed by additional thermal treatment in a protective silica shell allowing to enhance their magnetic properties. The cores are then isolated and covered de novo with a hybrid silica layer formed through the hydrolysis and polycondensation of tetraethoxysilane and a fluorescent silane synthesized from rhodamine, piperazine spacer, and 3-iodopropyltrimethoxysilane. The aminoalkyltrialkoxysilanes are strictly avoided and the resulting particles are hydrolytically stable and do not release dye. The high colloidal stability of the material and the long durability of the fluorescence are reinforced by an additional silica layer on the surface of the particles. Structural and magnetic studies of the products using XRD, TEM, and SQUID magnetometry confirm the importance of the thermal treatment and demonstrate that no mechanical treatment is required for the flux-synthesized manganite. Detailed cell viability tests show negligible or very low toxicity at concentrations at which excellent labeling is achieved. Predominant localization of nanoparticles in lysosomes is confirmed by immunofluorescence staining. Relaxometric and biological studies suggest that the functionalized nanoparticles are suitable for imaging applications.
Institute of Clinical and Experimental Medicine Vídeňská 1958 140 21 Praha 4 Czech Republic
Institute of Physics AS CR Cukrovarnická 10 162 00 Praha 6 Czech Republic
Laboratoire de Chimie UMR 5182 ENS CNRS UCBL 46 allée d'Italie 69364 Lyon cedex 07 France
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