Whole genome sequencing and phylogenetic analysis of feline anelloviruses
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25724090
DOI
10.1016/j.meegid.2015.02.015
PII: S1567-1348(15)00058-1
Knihovny.cz E-resources
- Keywords
- Anelloviruses, Cats, Genome sequencing, Prevalence,
- MeSH
- Phylogeny MeSH
- Genome, Viral genetics MeSH
- DNA Virus Infections epidemiology veterinary virology MeSH
- Cats virology MeSH
- Cat Diseases epidemiology virology MeSH
- Polymerase Chain Reaction veterinary MeSH
- Prevalence MeSH
- Torque teno virus genetics MeSH
- Viral Proteins genetics MeSH
- Animals MeSH
- Check Tag
- Cats virology MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- ORF1 protein, TT virus MeSH Browser
- Viral Proteins MeSH
Torque teno felis virus (FcTTV) was detected in the cat population in the Czech Republic. A total of 110 serum samples were tested by a nested PCR technique using specific primers, situated in the highly conserved untranslated region of the virus genome. The frequency of feline TT virus in the Czech Republic was found to be 33.63%. Sequencing of PCR product from several virus strains showed that all of them are closely related and belong to the same virus species. Whole genome sequencing of three strains was performed to compare overall genetic heterogeneity of feline TT viruses. One of these three strains showed more that 10% difference at the nucleotide level. Furthermore we didn't find any correlation between FcTTV infection and sex or health status of examined animals.
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