Cannabinoid-Induced Changes in the Activity of Electron Transport Chain Complexes of Brain Mitochondria
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25820672
DOI
10.1007/s12031-015-0545-2
PII: 10.1007/s12031-015-0545-2
Knihovny.cz E-resources
- MeSH
- Cannabinoid Receptor Antagonists pharmacology MeSH
- Citrate (si)-Synthase metabolism MeSH
- Cannabinoids pharmacology MeSH
- Mitochondria drug effects metabolism MeSH
- Brain drug effects metabolism MeSH
- Swine MeSH
- Electron Transport Complex I metabolism MeSH
- Electron Transport Complex II metabolism MeSH
- Electron Transport Complex III metabolism MeSH
- Electron Transport Complex IV metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cannabinoid Receptor Antagonists MeSH
- Citrate (si)-Synthase MeSH
- Cannabinoids MeSH
- Electron Transport Complex I MeSH
- Electron Transport Complex II MeSH
- Electron Transport Complex III MeSH
- Electron Transport Complex IV MeSH
The aim of this study was to investigate changes in the activity of individual mitochondrial respiratory chain complexes (I, II/III, IV) and citrate synthase induced by pharmacologically different cannabinoids. In vitro effects of selected cannabinoids on mitochondrial enzymes were measured in crude mitochondrial fraction isolated from pig brain. Both cannabinoid receptor agonists, Δ(9)-tetrahydrocannabinol, anandamide, and R-(+)-WIN55,212-2, and antagonist/inverse agonists of cannabinoid receptors, AM251, and cannabidiol were examined in pig brain mitochondria. Different effects of these cannabinoids on mitochondrial respiratory chain complexes and citrate synthase were found. Citrate synthase activity was decreased only by Δ(9)-tetrahydrocannabinol and AM251. Significant increase in the complex I activity was induced by anandamide. At micromolar concentration, all the tested cannabinoids inhibited the activity of electron transport chain complexes II/III and IV. Stimulatory effect of anandamide on activity of complex I may participate on distinct physiological effects of endocannabinoids compared to phytocannabinoids or synthetic cannabinoids. Common inhibitory effect of cannabinoids on activity of complex II/III and IV confirmed a non-receptor-mediated mechanism of cannabinoid action on individual components of system of oxidative phosphorylation.
See more in PubMed
Fertil Steril. 2009 Jun;91(6):2471-6 PubMed
Am J Physiol Lung Cell Mol Physiol. 2006 Jun;290(6):L1202-9 PubMed
Neurochem Int. 2010 Feb;56(3):394-403 PubMed
Cell Death Dis. 2013 Dec 05;4:e949 PubMed
Biochem Biophys Res Commun. 2005 Mar 11;328(2):623-32 PubMed
J Med Chem. 1998 Nov 19;41(24):4861-72 PubMed
Nat Neurosci. 2012 Mar 04;15(4):558-64 PubMed
Neuro Endocrinol Lett. 2010;31(5):645-56 PubMed
Biochem Pharmacol. 1972 May 1;21(9):1217-26 PubMed
Naunyn Schmiedebergs Arch Pharmacol. 2010 Jun;381(6):563-72 PubMed
J Neurosci. 2009 Feb 18;29(7):2053-63 PubMed
Am J Physiol Lung Cell Mol Physiol. 2003 Feb;284(2):L298-306 PubMed
Eur J Pharmacol. 2000 Apr 21;395(1):1-7 PubMed
Mol Psychiatry. 2005 Oct;10(10):900-19 PubMed
Life Sci. 1997;61(14 ):PL 191-7 PubMed
Mol Metab. 2014 Apr 02;3(4):495-504 PubMed
Eur J Pharmacol. 1996 Oct 10;313(1-2):17-24 PubMed
Toxicol Lett. 2014 Nov 18;231(1):62-71 PubMed
Clin Chim Acta. 1994 Jul;228(1):35-51 PubMed
Neuro Endocrinol Lett. 2010;31(3):336-42 PubMed
Pharmacology. 2010;85(6):328-35 PubMed
Addict Biol. 2007 Jun;12 (2):158-66 PubMed
Biochem Biophys Res Commun. 2007 Dec 7;364(1):131-7 PubMed
J Biol Chem. 1976 Aug 25;251(16):5002-6 PubMed
J Biol Chem. 1951 Nov;193(1):265-75 PubMed
Neurochem Res. 2011 Feb;36(2):327-38 PubMed
Methods Enzymol. 1996;264:484-509 PubMed
Biochem Biophys Res Commun. 2009 Oct 16;388(2):439-42 PubMed
