Cannabinoid-Induced Changes in the Activity of Electron Transport Chain Complexes of Brain Mitochondria
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25820672
DOI
10.1007/s12031-015-0545-2
PII: 10.1007/s12031-015-0545-2
Knihovny.cz E-zdroje
- MeSH
- antagonisté kanabinoidních receptorů farmakologie MeSH
- citrátsynthasa metabolismus MeSH
- kanabinoidy farmakologie MeSH
- mitochondrie účinky léků metabolismus MeSH
- mozek účinky léků metabolismus MeSH
- prasata MeSH
- respirační komplex I metabolismus MeSH
- respirační komplex II metabolismus MeSH
- respirační komplex III metabolismus MeSH
- respirační komplex IV metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antagonisté kanabinoidních receptorů MeSH
- citrátsynthasa MeSH
- kanabinoidy MeSH
- respirační komplex I MeSH
- respirační komplex II MeSH
- respirační komplex III MeSH
- respirační komplex IV MeSH
The aim of this study was to investigate changes in the activity of individual mitochondrial respiratory chain complexes (I, II/III, IV) and citrate synthase induced by pharmacologically different cannabinoids. In vitro effects of selected cannabinoids on mitochondrial enzymes were measured in crude mitochondrial fraction isolated from pig brain. Both cannabinoid receptor agonists, Δ(9)-tetrahydrocannabinol, anandamide, and R-(+)-WIN55,212-2, and antagonist/inverse agonists of cannabinoid receptors, AM251, and cannabidiol were examined in pig brain mitochondria. Different effects of these cannabinoids on mitochondrial respiratory chain complexes and citrate synthase were found. Citrate synthase activity was decreased only by Δ(9)-tetrahydrocannabinol and AM251. Significant increase in the complex I activity was induced by anandamide. At micromolar concentration, all the tested cannabinoids inhibited the activity of electron transport chain complexes II/III and IV. Stimulatory effect of anandamide on activity of complex I may participate on distinct physiological effects of endocannabinoids compared to phytocannabinoids or synthetic cannabinoids. Common inhibitory effect of cannabinoids on activity of complex II/III and IV confirmed a non-receptor-mediated mechanism of cannabinoid action on individual components of system of oxidative phosphorylation.
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