The effects of the F-actin inhibitor latrunculin A on the pathogenic yeast Cryptococcus neoformans
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25823986
DOI
10.1159/000377619
PII: 000377619
Knihovny.cz E-resources
- MeSH
- Actins antagonists & inhibitors MeSH
- Antifungal Agents pharmacology MeSH
- Bridged Bicyclo Compounds, Heterocyclic pharmacology MeSH
- Cell Wall drug effects metabolism MeSH
- Cell Division drug effects MeSH
- Cryptococcus neoformans drug effects metabolism MeSH
- Microscopy, Electron methods MeSH
- Microscopy, Fluorescence methods MeSH
- Cryptococcosis drug therapy metabolism microbiology MeSH
- Humans MeSH
- Actin Cytoskeleton drug effects metabolism MeSH
- Cell Proliferation drug effects MeSH
- Thiazolidines pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Antifungal Agents MeSH
- Bridged Bicyclo Compounds, Heterocyclic MeSH
- latrunculin A MeSH Browser
- Thiazolidines MeSH
BACKGROUND: This basic research aimed to investigate the effects of the actin inhibitor latrunculin A (LA) on the human pathogen Cryptococcus neoformans, by freeze-substitution (FS) and electron microscopy (EM), to determine whether the actin cytoskeleton can become a new antifungal target for inhibition of cell division. METHODS: Cells treated with LA for 20 h in yeast-extract peptone dextrose medium were investigated by phase-contrast and fluorescent microscopy, FS and transmission EM, counted in a Bürker chamber and the absorbance was then measured. RESULTS: The disappearance of actin patches, actin cables and actin rings demonstrated the response of the cells of C. neoformans to the presence of the actin inhibitor LA. The removal of actin cables and patches arrested proliferation and led to the production of cells that had ultrastructural disorder, irregular morphology of the mitochondria and thick aberrant cell walls. Budding cells lysed in the buds and septa. CONCLUSION: LA exerts fungistatic, fungicidal and fungilytic effects on the human pathogenic yeast C. neoformans.
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