Tobacco susceptibility to Potato virus Y(NTN) infection is affected by grafting and endogenous cytokinin content
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25900563
DOI
10.1016/j.plantsci.2015.02.017
PII: S0168-9452(15)00061-8
Knihovny.cz E-resources
- Keywords
- Antioxidant system, Cytokinin, Malic enzyme, Peroxidase, Salicylic acid, ipt gene,
- MeSH
- Chimera MeSH
- Cytokinins genetics metabolism MeSH
- Plants, Genetically Modified MeSH
- Host-Pathogen Interactions * MeSH
- Plant Roots MeSH
- Salicylic Acid metabolism MeSH
- Plant Leaves MeSH
- Plant Diseases * MeSH
- Disease Resistance * MeSH
- Hydrogen Peroxide metabolism MeSH
- Peroxidase metabolism MeSH
- Potyvirus * MeSH
- Plant Growth Regulators metabolism MeSH
- Solanum tuberosum MeSH
- Nicotiana genetics metabolism virology MeSH
- Agriculture methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytokinins MeSH
- Salicylic Acid MeSH
- Hydrogen Peroxide MeSH
- Peroxidase MeSH
- Plant Growth Regulators MeSH
Faster or stronger response to pathogen occurs if plants undergo prior priming. Cytokinins seem to be also involved in plant priming and in response to pathogens. Susceptibility to Potato virus Y(NTN) (PVY(NTN)) was studied in transgenic cytokinin overproducing (Pssu-ipt) tobacco and compared with nontransgenic plants. Since cytokinin overproduction inhibits development of plant roots and grafting overcomes this limitation, both types were grown as rooted and/or grafted plants to check also the effect of grafting. Control rooted tobacco (C), the most susceptible to PVY(NTN), showed always symptoms during the infection together with the rising virus content and a systemic response, such as accumulation of H2O2, salicylic acid (SA) and other phenolic acids, and stress-induced enzyme activities. In transgenic and grafted plants, the response to PVY(NTN) was dependent on protective mechanisms activated prior to the inoculation. In Pssu-ipt tobacco, cytokinin active forms and SA contents exceeded manifold their content in C. Grafting promoted the accumulation of phenolics, but SA, and stimulated peroxidase activities. Thus, the pre-infection barrier established in both transgenic and grafted plants helped to suppress partly the virus multiplication and resulted in milder symptom development. However, only the synergic effect of both grafting and the high cytokinins led to PVY(NTN) tolerance in transgenic grafts. Possible mechanisms were discussed.
References provided by Crossref.org
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