Phthalocyanine-mediated Photodynamic Treatment of Tumoural and Non-tumoural cell lines
Language English Country Greece Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26124341
PII: 35/7/3943
Knihovny.cz E-resources
- Keywords
- Photosensitizer, Phthalocyanine, cellular damage, oxidative stress, photodynamic treatment, phototoxicity, tumour cells,
- MeSH
- Cell Line MeSH
- NIH 3T3 Cells MeSH
- Photochemotherapy methods MeSH
- Photosensitizing Agents pharmacology MeSH
- HeLa Cells MeSH
- Magnesium pharmacology MeSH
- Indoles pharmacology MeSH
- Isoindoles MeSH
- Humans MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Neoplasms drug therapy metabolism MeSH
- Reactive Oxygen Species metabolism MeSH
- Zinc pharmacology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Photosensitizing Agents MeSH
- Magnesium MeSH
- Indoles MeSH
- Isoindoles MeSH
- phthalocyanine MeSH Browser
- Reactive Oxygen Species MeSH
- Zinc MeSH
This study deals with the use of cationic far-red absorbing photosensitizers (λ(max) ~740 nm) from the group of the phthalocyanines, in photodynamic therapy. The photosensitizers differed in their central atom, bearing either hydrogen, zinc or magnesium. These photosensitizers were tested in vitro on the tumour cell line HeLa (cervical cancer) and non-tumour cell line NIH3T3 (mouse fibroblast). The following tests were performed: measurement of reactive oxygen species production, viability testing, Comet assay and cell type detection (apoptotic, necrotic and living cells). The best results were achieved with zinc derivative at relatively low half-maximum inhibitory concentration (0.04 μM) and a total radiation dose of 15 J cm(-2).
Differences in the Effects of Broad-Band UVA and Narrow-Band UVB on Epidermal Keratinocytes
