Phthalocyanine-mediated Photodynamic Treatment of Tumoural and Non-tumoural cell lines
Jazyk angličtina Země Řecko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26124341
PII: 35/7/3943
Knihovny.cz E-zdroje
- Klíčová slova
- Photosensitizer, Phthalocyanine, cellular damage, oxidative stress, photodynamic treatment, phototoxicity, tumour cells,
- MeSH
- buněčné linie MeSH
- buňky NIH 3T3 MeSH
- fotochemoterapie metody MeSH
- fotosenzibilizující látky farmakologie MeSH
- HeLa buňky MeSH
- hořčík farmakologie MeSH
- indoly farmakologie MeSH
- isoindoly MeSH
- lidé MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- nádory farmakoterapie metabolismus MeSH
- reaktivní formy kyslíku metabolismus MeSH
- zinek farmakologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fotosenzibilizující látky MeSH
- hořčík MeSH
- indoly MeSH
- isoindoly MeSH
- phthalocyanine MeSH Prohlížeč
- reaktivní formy kyslíku MeSH
- zinek MeSH
This study deals with the use of cationic far-red absorbing photosensitizers (λ(max) ~740 nm) from the group of the phthalocyanines, in photodynamic therapy. The photosensitizers differed in their central atom, bearing either hydrogen, zinc or magnesium. These photosensitizers were tested in vitro on the tumour cell line HeLa (cervical cancer) and non-tumour cell line NIH3T3 (mouse fibroblast). The following tests were performed: measurement of reactive oxygen species production, viability testing, Comet assay and cell type detection (apoptotic, necrotic and living cells). The best results were achieved with zinc derivative at relatively low half-maximum inhibitory concentration (0.04 μM) and a total radiation dose of 15 J cm(-2).
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