PLK1 regulates spindle formation kinetics and APC/C activation in mouse zygote
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26174739
DOI
10.1017/s0967199415000246
PII: S0967199415000246
Knihovny.cz E-zdroje
- Klíčová slova
- APC/C, BI2536, Live cell imaging, Mouse zygote, PLK1, Securin, Spindle assembly,
- MeSH
- anafáze MeSH
- anafázi podporující komplex metabolismus MeSH
- aparát dělícího vřeténka metabolismus MeSH
- blastocysta MeSH
- časosběrné zobrazování MeSH
- centrozom metabolismus MeSH
- kinetika MeSH
- kinetochory metabolismus MeSH
- konfokální mikroskopie MeSH
- mitóza MeSH
- myši MeSH
- polo-like kinasa 1 MeSH
- protein-serin-threoninkinasy antagonisté a inhibitory metabolismus MeSH
- proteiny buněčného cyklu antagonisté a inhibitory metabolismus MeSH
- protoonkogenní proteiny antagonisté a inhibitory metabolismus MeSH
- pteridiny farmakologie MeSH
- zvířata MeSH
- zygota účinky léků metabolismus MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- anafázi podporující komplex MeSH
- BI 2536 MeSH Prohlížeč
- protein-serin-threoninkinasy MeSH
- proteiny buněčného cyklu MeSH
- protoonkogenní proteiny MeSH
- pteridiny MeSH
Polo-like kinase 1 (PLK1) is involved in essential events of cell cycle including mitosis in which it participates in centrosomal microtubule nucleation, spindle bipolarity establishment and cytokinesis. Although PLK1 function has been studied in cycling cancer cells, only limited data are known about its role in the first mitosis of mammalian zygotes. During the 1-cell stage of mouse embryo development, the acentriolar spindle is formed and the shift from acentriolar to centrosomal spindle formation progresses gradually throughout the preimplantation stage, thus providing a unique possibility to study acentriolar spindle formation. We have shown previously that PLK1 activity is not essential for entry into first mitosis, but is required for correct spindle formation and anaphase onset in 1-cell mouse embryos. In the present study, we extend this knowledge by employing quantitative confocal live cell imaging to determine spindle formation kinetics in the absence of PLK1 activity and answer the question whether metaphase arrest at PLK1-inhibited embryos is associated with low anaphase-promoting complex/cyclosome (APC/C) activity and consequently high securin level. We have shown that inhibition of PLK1 activity induces a delay in onset of acentriolar spindle formation during first mitosis. Although these PLK1-inhibited 1-cell embryos were finally able to form a bipolar spindle, not all chromosomes were aligned at the metaphase equator. PLK1-inhibited embryos were arrested in metaphase without any sign of APC/C activation with high securin levels. Our results document that PLK1 controls the onset of spindle assembly and spindle formation, and is essential for APC/C activation before anaphase onset in mouse zygotes.
Institute of Animal Physiology Slovak Academy of Sciences Kosice Slovakia
Institute of Animal Physiology Slovak Academy of Sciences Soltesovej 4 040 01 Kosice Slovakia
Citace poskytuje Crossref.org
Multiple Roles of PLK1 in Mitosis and Meiosis
The neglected part of early embryonic development: maternal protein degradation
