The Effect of Ascorbic Acid on Mancozeb-Induced Toxicity in Rat Thymocytes
Language English Country Czech Republic Media print
Document type Evaluation Study, Journal Article
PubMed
26213857
DOI
10.14712/fb2015061030116
PII: file/5781/fb2015a0016.pdf
Knihovny.cz E-resources
- MeSH
- Adenosine Triphosphate metabolism MeSH
- Antioxidants administration & dosage pharmacology MeSH
- Apoptosis drug effects MeSH
- Biomarkers metabolism MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Ascorbic Acid administration & dosage pharmacology MeSH
- Maneb administration & dosage toxicity MeSH
- Membrane Potential, Mitochondrial drug effects MeSH
- Oxidative Stress drug effects MeSH
- Rats, Wistar MeSH
- Fungicides, Industrial administration & dosage toxicity MeSH
- Reactive Oxygen Species metabolism MeSH
- Thymocytes drug effects metabolism MeSH
- Cell Survival drug effects MeSH
- Zineb administration & dosage toxicity MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Names of Substances
- Adenosine Triphosphate MeSH
- Antioxidants MeSH
- Biomarkers MeSH
- Ascorbic Acid MeSH
- mancozeb MeSH Browser
- Maneb MeSH
- Fungicides, Industrial MeSH
- Reactive Oxygen Species MeSH
- Zineb MeSH
Mancozeb, as a dithiocarbamate fungicide, has been found to exhibit toxicological manifestations in different cells, mainly by generation of free radicals which may alter antioxidant defence systems in cells. The effect of mancozeb on the cells of a primary lymphoid organ has not been studied. In the present study, the effects of mancozeb (0.2, 2 and 5 μg/ml) or mancozeb+ascorbic acid (100 μg/ml), or ascorbic acid alone or control medium alone on the levels of cell viability, apoptosis, intracellular reactive oxygen species production (ROS), mitochondrial membrane potential (MMP) and ATP levels in rat thymocytes were examined in vitro. Cells treated with mancozeb displayed a concentration-dependent increase of hypodiploid cells and ROS production followed by markedly decreased viability of the cells, MMP and ATP levels. Application of ascorbic acid significantly reduced cytotoxicity in cell cultures treated with 0.2 and 2 μg/ml of mancozeb, together with significantly decreased ROS levels and increased MMP and ATP levels. In cells treated with 5 μg/ml of mancozeb, ascorbic acid failed to reduce toxicity while simultaneously increasing the apoptosis rate of thymocytes. These results suggest that ROS plays a significant role in mancozeb-induced toxicity, through alteration of mitochondrial function. Ascorbic acid administration reduced the toxicity rate in cells treated with lower mancozeb concentrations, while it may have the ability to shift cells from necrosis to apoptosis in the presence of highest mancozeb concentrations.
Clinic for Gynaecology and Obstetrics University Clinical Centre Nis Serbia
Institute of Physiology Medical Faculty University of Nis Serbia
References provided by Crossref.org