Regulatory Impact of Amniotic Membrane Transplantation on Presence of Adhesion/Growth-Regulatory Galectins-1 and -7 in Corneal Explants from Acanthamoeba Keratitis Patients: Clinical Note
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, multicentrická studie, práce podpořená grantem
- Klíčová slova
- Agglutinin, cornea, immunohistochemistry, keratoplasty, lectin,
- MeSH
- akantamébová keratitida metabolismus chirurgie MeSH
- amnion transplantace MeSH
- biologické krytí * MeSH
- biologické markery metabolismus MeSH
- dospělí MeSH
- galektin 1 MeSH
- galektiny metabolismus MeSH
- imunohistochemie MeSH
- keratoplastika perforující metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- oční infekce parazitární diagnóza metabolismus chirurgie MeSH
- rohovka metabolismus chirurgie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biologické markery MeSH
- galektin 1 MeSH
- galektiny MeSH
- LGALS7 protein, human MeSH Prohlížeč
PURPOSE: To assess the impact of Acanthamoeba keratitis (AK) and amniotic membrane transplantation (AMT) in corneal explants on presence of two multifunctional endogenous lectins, i.e. galectins-1 and -7. METHODS: Ten corneal explants from AK patients (five with previous AMT and five controls without this treatment) and seven specimens of disease-free control cornea were processed by indirect fluorescent immunohistochemistry. RESULTS: Immunostaining for both galectins was obtained in the epithelium, stroma and the endothelial layer of all controls, with the strongest positivity in the epithelium. Significantly decreased intensity for galectin-1 was recorded in the epithelium of corneal explants from patients with AK and AMT. The signal for galectin-7 was significantly decreased in the epithelium of AK patients and normalized after AMT. CONCLUSIONS: AMT has a marked impact on presence of the two galectins in opposite directions, encouraging complete profiling for this family of endogenous effectors.
Department of Ophthalmology 1st Faculty of Medicine Charles University Prague U Nemocnice Prague
e Lexum European Eye Clinic Antala Staška Prague Czech Republic and
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