Regulatory Impact of Amniotic Membrane Transplantation on Presence of Adhesion/Growth-Regulatory Galectins-1 and -7 in Corneal Explants from Acanthamoeba Keratitis Patients: Clinical Note
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't
- Keywords
- Agglutinin, cornea, immunohistochemistry, keratoplasty, lectin,
- MeSH
- Acanthamoeba Keratitis metabolism surgery MeSH
- Amnion transplantation MeSH
- Biological Dressings * MeSH
- Biomarkers metabolism MeSH
- Adult MeSH
- Galectin 1 MeSH
- Galectins metabolism MeSH
- Immunohistochemistry MeSH
- Keratoplasty, Penetrating methods MeSH
- Middle Aged MeSH
- Humans MeSH
- Eye Infections, Parasitic diagnosis metabolism surgery MeSH
- Cornea metabolism surgery MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Multicenter Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- Galectin 1 MeSH
- Galectins MeSH
- LGALS7 protein, human MeSH Browser
PURPOSE: To assess the impact of Acanthamoeba keratitis (AK) and amniotic membrane transplantation (AMT) in corneal explants on presence of two multifunctional endogenous lectins, i.e. galectins-1 and -7. METHODS: Ten corneal explants from AK patients (five with previous AMT and five controls without this treatment) and seven specimens of disease-free control cornea were processed by indirect fluorescent immunohistochemistry. RESULTS: Immunostaining for both galectins was obtained in the epithelium, stroma and the endothelial layer of all controls, with the strongest positivity in the epithelium. Significantly decreased intensity for galectin-1 was recorded in the epithelium of corneal explants from patients with AK and AMT. The signal for galectin-7 was significantly decreased in the epithelium of AK patients and normalized after AMT. CONCLUSIONS: AMT has a marked impact on presence of the two galectins in opposite directions, encouraging complete profiling for this family of endogenous effectors.
Department of Ophthalmology 1st Faculty of Medicine Charles University Prague U Nemocnice Prague
e Lexum European Eye Clinic Antala Staška Prague Czech Republic and
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