PURPOSE: To assess the impact of Acanthamoeba keratitis (AK) and amniotic membrane transplantation (AMT) in corneal explants on presence of two multifunctional endogenous lectins, i.e. galectins-1 and -7. METHODS: Ten corneal explants from AK patients (five with previous AMT and five controls without this treatment) and seven specimens of disease-free control cornea were processed by indirect fluorescent immunohistochemistry. RESULTS: Immunostaining for both galectins was obtained in the epithelium, stroma and the endothelial layer of all controls, with the strongest positivity in the epithelium. Significantly decreased intensity for galectin-1 was recorded in the epithelium of corneal explants from patients with AK and AMT. The signal for galectin-7 was significantly decreased in the epithelium of AK patients and normalized after AMT. CONCLUSIONS: AMT has a marked impact on presence of the two galectins in opposite directions, encouraging complete profiling for this family of endogenous effectors.

• MeSH
• akantamébová keratitida metabolismus   chirurgie   MeSH
• amnion transplantace   MeSH
• biologické krytí * MeSH
• biologické markery metabolismus   MeSH
• dospělí MeSH
• galektin 1 MeSH
• galektiny metabolismus   MeSH
• imunohistochemie MeSH
• keratoplastika perforující metody   MeSH
• lidé středního věku MeSH
• lidé MeSH
• oční infekce parazitární diagnóza   metabolismus   chirurgie   MeSH
• rohovka metabolismus   chirurgie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• multicentrická studie MeSH