A Conserved Cytochrome P450 Evolved in Seed Plants Regulates Flower Maturation
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
BB/E006922/1
Biotechnology and Biological Sciences Research Council - United Kingdom
BBS/E/C/00004951
Biotechnology and Biological Sciences Research Council - United Kingdom
PubMed
26388305
DOI
10.1016/j.molp.2015.09.002
PII: S1674-2052(15)00363-9
Knihovny.cz E-resources
- Keywords
- flower development, gibberellins, jasmonate, negative selection, phylogenomics, volatile compounds,
- MeSH
- Arabidopsis classification enzymology genetics growth & development MeSH
- Phylogeny MeSH
- Conserved Sequence MeSH
- Flowers classification enzymology genetics growth & development MeSH
- Arabidopsis Proteins chemistry genetics metabolism MeSH
- Gene Expression Regulation, Plant MeSH
- Plants classification enzymology genetics MeSH
- Seeds classification enzymology genetics growth & development MeSH
- Cytochrome P-450 Enzyme System chemistry genetics metabolism MeSH
- Gene Expression Regulation, Developmental MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- cytochrome P-450 715A1, Arabidopsis MeSH Browser
- Arabidopsis Proteins MeSH
- Cytochrome P-450 Enzyme System MeSH
Global inspection of plant genomes identifies genes maintained in low copies across taxa and under strong purifying selection, which are likely to have essential functions. Based on this rationale, we investigated the function of the low-duplicated CYP715 cytochrome P450 gene family that appeared early in seed plants and evolved under strong negative selection. Arabidopsis CYP715A1 showed a restricted tissue-specific expression in the tapetum of flower buds and in the anther filaments upon anthesis. cyp715a1 insertion lines showed a strong defect in petal development, and transient alteration of pollen intine deposition. Comparative expression analysis revealed the downregulated expression of genes involved in pollen development, cell wall biogenesis, hormone homeostasis, and floral sesquiterpene biosynthesis, especially TPS21 and several key genes regulating floral development such as MYB21, MYB24, and MYC2. Accordingly, floral sesquiterpene emission was suppressed in the cyp715a1 mutants. Flower hormone profiling, in addition, indicated a modification of gibberellin homeostasis and a strong disturbance of the turnover of jasmonic acid derivatives. Petal growth was partially restored by the active gibberellin GA3 or the functional analog of jasmonoyl-isoleucine, coronatine. CYP715 appears to function as a key regulator of flower maturation, synchronizing petal expansion and volatile emission. It is thus expected to be an important determinant of flower-insect interaction.
Graduate School of Agriculture Shizuoka University Shizuoka 422 8529 Japan
Laboratoire de Biométrie et Biologie Évolutive Université Lyon 1 CNRS 69622 Villeurbanne France
Plateforme d'Imagerie In Vitro IFR 37 de Neurosciences 67084 Strasbourg France
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