Hormonal and epigenetic regulation during embryogenic tissue habituation in Cucurbita pepo L
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26403461
DOI
10.1007/s00299-015-1869-7
PII: 10.1007/s00299-015-1869-7
Knihovny.cz E-resources
- Keywords
- ABA, CKs, DNA methylation, Habituation, IAA, Somatic embryogenesis,
- MeSH
- Azacitidine pharmacology MeSH
- Cucurbita drug effects embryology genetics MeSH
- Cytokinins metabolism MeSH
- Epigenesis, Genetic drug effects MeSH
- Herbicides pharmacology MeSH
- Enzyme Inhibitors pharmacology MeSH
- 2,4-Dichlorophenoxyacetic Acid pharmacology MeSH
- Abscisic Acid metabolism MeSH
- Indoleacetic Acids metabolism MeSH
- DNA Methylation drug effects MeSH
- Plant Growth Regulators metabolism MeSH
- Plant Somatic Embryogenesis Techniques MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Azacitidine MeSH
- Cytokinins MeSH
- Herbicides MeSH
- indoleacetic acid MeSH Browser
- Enzyme Inhibitors MeSH
- 2,4-Dichlorophenoxyacetic Acid MeSH
- Abscisic Acid MeSH
- Indoleacetic Acids MeSH
- Plant Growth Regulators MeSH
Habituated embryogenic line of pumpkin contained more CKs and IAA, but less ABA than the non-habituated line. Pronounced hypomethylation correlated with the absence of 2,4-D, addition of 5-azaC, and the process of habituation. A comparative analysis between habituated and non-habituated embryogenic cultures of pumpkin (Cucurbita pepo L.) in relation to endogenous phytohormones, global DNA methylation, and developmental and regeneration capacities of the cultures was conducted. The analysis revealed more cytokinins (CKs) and indole-3-acetic acid (IAA), but less abscisic acid (ABA) in the habituated HEC line than in the non-habituated DEC line. Ribosides and ribotides were the most abundant CK forms in both HEC and DEC lines (75.9 and 57.6 %, respectively). HEC contained more free-base CKs (5.8 vs. 3.2 %), whereas DEC contained considerably more O-glycosides (39.1 vs. 18.3 %). Although prevalence of IAA was common for both lines, relative ratio of CKs and ABA differed between DEC and HEC lines. ABA was prevailing over CKs in DEC, while CKs prevailed over ABA in HEC line. Taking into account the importance of ABA for embryo maturation, the reduced endogenous ABA content in HEC line might be the reason for a 5-fold reduction in regeneration capacity compared to DEC. Both habituated and non-habituated embryogenic lines were highly methylated in the presence of 2,4-dichlorophenoxyacetic acid (2,4-D). Pronounced hypomethylation correlated with the absence of 2,4-D, addition of 5-azacytidine (5-azaC), but also with the process of habituation. The habituated line was resistant to the effect of hypomethylation drug 5-azaC and remained highly methylated even after the addition of 5-azaC. Also, 5-azaC did not change the developmental pattern in the habituated line, indicating the existence of separate mechanisms by which 2,4-D influences global DNA methylation in comparison to habituation-related global DNA methylation.
Faculty of Science University of Zagreb Horvatovac 102a 10000 Zagreb Croatia
Institute Ruđer Bošković Bijenička cesta 54 10000 Zagreb Croatia
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