Ischemic Postconditioning and Nitric Oxide Administration Failed to Confer Protective Effects in a Porcine Model of Extracorporeal Cardiopulmonary Resuscitation
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26412075
DOI
10.1111/aor.12556
Knihovny.cz E-zdroje
- Klíčová slova
- Cardiac arrest, Cerebral oxygenation, Extracorporeal cardiopulmonary resuscitation, Hypothermia, Ischemic postconditioning, Nitric oxide, Organ protection,
- MeSH
- alanintransaminasa krev MeSH
- cystatin C krev MeSH
- fosfopyruváthydratasa krev MeSH
- ischemický postconditioning metody MeSH
- kardiopulmonální resuscitace metody MeSH
- kreatinkinasa krev MeSH
- krevní tlak MeSH
- mimotělní membránová oxygenace metody MeSH
- modely nemocí na zvířatech MeSH
- myoglobin krev MeSH
- ochranné látky terapeutické užití MeSH
- oxid dusnatý terapeutické užití MeSH
- oxidační stres MeSH
- prasata MeSH
- reaktivní formy kyslíku krev MeSH
- troponin I krev MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alanintransaminasa MeSH
- cystatin C MeSH
- fosfopyruváthydratasa MeSH
- kreatinkinasa MeSH
- myoglobin MeSH
- ochranné látky MeSH
- oxid dusnatý MeSH
- reaktivní formy kyslíku MeSH
- troponin I MeSH
The protective effects of ischemic postconditioning (IPC) and nitric oxide (NO) administration have been demonstrated in several ischemic scenarios. However, current evidence regarding the effect of IPC and NO in extracorporeal cardiopulmonary resuscitation remains lacking. Fifteen female swine (body weight 45 kg) underwent veno-arterial extracorporeal membrane oxygenation (ECMO) implantation; cardiac arrest-ventricular fibrillation was induced by rapid ventricular pacing. After 20 min of cardiac arrest, blood flow was restored by increasing the ECMO flow rate to 4.5 L/min. The animals (five per group) were then randomly assigned to receive IPC (three cycles of 3 min ischemia and reperfusion), NO (80 ppm via oxygenator), or mild hypothermia (HT; 33.0°C). Cerebral oximetry and aortic blood pressure were monitored continuously. After 90 min of reperfusion, blood samples were drawn for the measurement of troponin I, myoglobin, creatine-phosphokinase, alanine aminotransferase, neuron-specific enolase, cystatin C, and reactive oxygen metabolite (ROM) levels. Significantly higher blood pressure and cerebral oxygen saturation values were observed in the HT group compared with the IPC and NO groups (P < 0.05). The levels of troponin I, myoglobin, creatine phosphokinase, and alanine aminotransferase were significantly lower in the HT group (P < 0.05); levels of neuron-specific enolase, cystatin C, and ROM were not significantly different. IPC and NO were comparable in all monitored parameters. The results of the present study indicate that IPC and NO administration are not superior interventions to HT for the maintenance of blood pressure, cerebral oxygenation, organ protection, and suppression of oxidative stress following extracorporeal cardiopulmonary resuscitation.
Cardiovascular Center Na Homolce Hospital Prague Czech Republic
Department of Nuclear Medicine Na Homolce Hospital Prague Czech Republic
Department of Physiology 1st Faculty of Medicine Charles University Prague Prague Czech Republic
Faculty of Biomedical Engineering Czech Technical University Prague Prague Czech Republic
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