Targeted Mutagenesis in Bombyx mori Using TALENs
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Engineered nucleases, Golden Gate assembly, NHEJ, Nonhomologous end joining, Silkworm, pBlue-TAL,
- MeSH
- Bombyx genetics MeSH
- DNA-Binding Proteins genetics MeSH
- Endonucleases chemistry genetics MeSH
- Animals, Genetically Modified MeSH
- Genome, Insect MeSH
- Gene Targeting methods MeSH
- Humans MeSH
- Mutagenesis MeSH
- Trans-Activators genetics MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA-Binding Proteins MeSH
- Endonucleases MeSH
- Trans-Activators MeSH
Bombyx mori is a valuable model organism of high economic importance. Its genome sequence is available, as well as basic genetic and molecular genetic tools and markers. The introduction of genome editing methods based on engineered nucleases enables precise manipulations with genomic DNA, including targeted DNA deletions, insertions, or replacements in the genome allowing gene analysis and various applications. We describe here the use of TALENs which have a simple modular design of their DNA-binding domains, are easy to prepare and proved to be efficient in targeting of a wide range of cleavage sites. Our procedure often allows the production of individuals carrying homozygous mutations as early as in the G1 generation.
Biology Centre Czech Academy of Sciences Branisovska 31 370 05 Ceske Budejovice Czech Republic
National Institute of Agrobiological Sciences 1 2 Owashi Tsukuba Ibaraki 305 8634 Japan
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