Targeted Mutagenesis in Bombyx mori Using TALENs
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Engineered nucleases, Golden Gate assembly, NHEJ, Nonhomologous end joining, Silkworm, pBlue-TAL,
- MeSH
- bourec genetika MeSH
- DNA vazebné proteiny genetika MeSH
- endonukleasy chemie genetika MeSH
- geneticky modifikovaná zvířata MeSH
- genom hmyzu MeSH
- genový targeting metody MeSH
- lidé MeSH
- mutageneze MeSH
- trans-aktivátory genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA vazebné proteiny MeSH
- endonukleasy MeSH
- trans-aktivátory MeSH
Bombyx mori is a valuable model organism of high economic importance. Its genome sequence is available, as well as basic genetic and molecular genetic tools and markers. The introduction of genome editing methods based on engineered nucleases enables precise manipulations with genomic DNA, including targeted DNA deletions, insertions, or replacements in the genome allowing gene analysis and various applications. We describe here the use of TALENs which have a simple modular design of their DNA-binding domains, are easy to prepare and proved to be efficient in targeting of a wide range of cleavage sites. Our procedure often allows the production of individuals carrying homozygous mutations as early as in the G1 generation.
Biology Centre Czech Academy of Sciences Branisovska 31 370 05 Ceske Budejovice Czech Republic
National Institute of Agrobiological Sciences 1 2 Owashi Tsukuba Ibaraki 305 8634 Japan
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