Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26463367
DOI
10.1016/j.bmc.2015.09.051
PII: S0968-0896(15)30076-6
Knihovny.cz E-zdroje
- Klíčová slova
- DNA, DNA polymerase, Modified nucleotides, Pyrimidine nucleosides, Restriction endonucleases,
- MeSH
- DNA analýza metabolismus MeSH
- flucytosin chemie MeSH
- fluoruracil chemie MeSH
- restrikční endonukleasy typu II metabolismus MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- štěpení DNA MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA MeSH
- flucytosin MeSH
- fluoruracil MeSH
- restrikční endonukleasy typu II MeSH
A systematic study of the cleavage of DNA sequences containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases (REs) was performed and the results compared with the same sequences containing natural pyrimidine bases, uracil or 5-methylcytosine. The results show that some REs recognize fluorine as a hydrogen on cytosine and cleave the corresponding sequences where the presence of m5dC leads to blocking of the cleavage. However, on uracil, the same REs recognize the F as a methyl surrogate and cleave the sequences which are not cleaved if uracil is incorporated instead of thymine. These results are interesting for understanding the recognition of DNA sequences by REs and for manipulation of the specific DNA cutting.
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