Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26463367
DOI
10.1016/j.bmc.2015.09.051
PII: S0968-0896(15)30076-6
Knihovny.cz E-resources
- Keywords
- DNA, DNA polymerase, Modified nucleotides, Pyrimidine nucleosides, Restriction endonucleases,
- MeSH
- DNA analysis metabolism MeSH
- Flucytosine chemistry MeSH
- Fluorouracil chemistry MeSH
- Deoxyribonucleases, Type II Site-Specific metabolism MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- DNA Cleavage MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA MeSH
- Flucytosine MeSH
- Fluorouracil MeSH
- Deoxyribonucleases, Type II Site-Specific MeSH
A systematic study of the cleavage of DNA sequences containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases (REs) was performed and the results compared with the same sequences containing natural pyrimidine bases, uracil or 5-methylcytosine. The results show that some REs recognize fluorine as a hydrogen on cytosine and cleave the corresponding sequences where the presence of m5dC leads to blocking of the cleavage. However, on uracil, the same REs recognize the F as a methyl surrogate and cleave the sequences which are not cleaved if uracil is incorporated instead of thymine. These results are interesting for understanding the recognition of DNA sequences by REs and for manipulation of the specific DNA cutting.
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