• This record comes from PubMed

Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases

. 2015 Nov 01 ; 23 (21) : 6885-90. [epub] 20151014

Language English Country England, Great Britain Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Links

PubMed 26463367
DOI 10.1016/j.bmc.2015.09.051
PII: S0968-0896(15)30076-6
Knihovny.cz E-resources

A systematic study of the cleavage of DNA sequences containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases (REs) was performed and the results compared with the same sequences containing natural pyrimidine bases, uracil or 5-methylcytosine. The results show that some REs recognize fluorine as a hydrogen on cytosine and cleave the corresponding sequences where the presence of m5dC leads to blocking of the cleavage. However, on uracil, the same REs recognize the F as a methyl surrogate and cleave the sequences which are not cleaved if uracil is incorporated instead of thymine. These results are interesting for understanding the recognition of DNA sequences by REs and for manipulation of the specific DNA cutting.

References provided by Crossref.org

Find record

Citation metrics

Loading data ...

Archiving options

Loading data ...