BACKGROUND AND AIMS: Polyploidy in plants has been studied extensively. In many groups, two or more cytotypes represent separate biological entities with distinct distributions, histories and ecology. This study examines the distribution and origins of cytotypes of Alnus glutinosa in Europe, North Africa and western Asia. METHODS: A combined approach was used involving flow cytometry and microsatellite analysis of 12 loci in 2200 plants from 209 populations combined with species distribution modelling using MIROC and CCSM climatic models, in order to analyse (1) ploidy and genetic variation, (2) the origin of tetraploid A. glutinosa, considering A. incana as a putative parent, and (3) past distributions of the species. KEY RESULTS: The occurrence of tetraploid populations of A. glutinosa in Europe is determined for the first time. The distribution of tetraploids is far from random, forming two geographically well-delimited clusters located in the Iberian Peninsula and the Dinaric Alps. Based on microsatellite analysis, both tetraploid clusters are probably of autopolyploid origin, with no indication that A. incana was involved in their evolutionary history. A projection of the MIROC distribution model into the Last Glacial Maximum (LGM) showed that (1) populations occurring in the Iberian Peninsula and North Africa were probably interconnected during the LGM and (2) populations occurring in the Dinaric Alps did not exist throughout the last glacial periods, having retreated southwards into lowland areas of the Balkan Peninsula. CONCLUSIONS: Newly discovered tetraploid populations are situated in the putative main glacial refugia, and neither of them was likely to have been involved in the colonization of central and northern Europe after glacial withdrawal. This could mean that neither the Iberian Peninsula nor the western part of the Balkan Peninsula served as effective refugial areas for northward post-glacial expansion of A. glutinosa.

Institute of Botany The Czech Academy of Sciences Zámek 1 CZ 252 43 Průhonice Czech Republic;

Institute of Botany The Czech Academy of Sciences Zámek 1 CZ 252 43 Průhonice Czech Republic; Faculty of Agriculture University of South Bohemia Studentská 13 CZ 370 05 České Budějovice Czech Republic and

Institute of Botany The Czech Academy of Sciences Zámek 1 CZ 252 43 Průhonice Czech Republic; Faculty of Environmental Sciences Czech University of Life Sciences Prague Kamýcká 129 Praha 6 Suchdol CZ 165 21 Czech Republic;

University of Pau and Pays Adour UFR Sciences et Techniques Departement de Biologie F 64100 Pau France

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