A novel surfactant-, NaCl-, and protease-tolerant β-mannanase from Bacillus sp. HJ14
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26489953
DOI
10.1007/s12223-015-0430-y
PII: 10.1007/s12223-015-0430-y
Knihovny.cz E-zdroje
- MeSH
- aktivace enzymů účinky léků MeSH
- Bacillus účinky léků genetika metabolismus MeSH
- beta-mannosidasa chemie genetika izolace a purifikace metabolismus MeSH
- chlorid sodný farmakologie MeSH
- detergenty farmakologie MeSH
- endopeptidasy metabolismus MeSH
- exprese genu MeSH
- genom bakteriální MeSH
- hydrolýza MeSH
- ionty MeSH
- kovy MeSH
- povrchově aktivní látky farmakologie MeSH
- rekombinantní proteiny MeSH
- sekvence aminokyselin MeSH
- sekvenční analýza DNA MeSH
- stabilita enzymů účinky léků MeSH
- tolerance k soli * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- beta-mannosidasa MeSH
- chlorid sodný MeSH
- detergenty MeSH
- endopeptidasy MeSH
- ionty MeSH
- kovy MeSH
- povrchově aktivní látky MeSH
- rekombinantní proteiny MeSH
A glycoside hydrolase family 5 β-mannanase-encoding gene was cloned from Bacillus sp. HJ14 isolated from saline soil in Heijing town. Coding sequence of mature protein (without the predicted signal peptide from M1 to A30) was successfully expressed in Escherichia coli BL21 (DE3). Purified recombinant mannanase (rMan5HJ14) exhibited optimal activity at pH 6.5 and 65 °C. The enzyme showed good salt tolerance, retaining more than 56 % β-mannanase activity at 3.0-30.0 % (w/v) NaCl and more than 94 % of the initial activity after incubation with 3.0-30.0 % (w/v) NaCl at 37 °C for 60 min. Almost no mannanase activity was lost after incubation of rMan5HJ14 with trypsin, proteinase K, and Alcalase at 37 °C for 60 min. Surfactants and chelating agents, namely SDS, CTAB, Tween 80, Triton X-100, EDTA, and sodium tripolyphosphate, showed little or no effect (retaining >82.4 % activity) on enzymatic activity. Liquid detergents, namely Tupperware, Walch, Bluemoon, Tide, and OMO, also showed little or no effect (retaining >72.4 % activity) on enzymatic activity at 0.5-2.0 % (v/v). The enzyme further presents a high proportion (11.97 %) of acidic amino acid residues (D and E), which may affect the SDS and NaCl tolerance of the enzyme. Together, the mannanase may be an alternative for potential use in liquid detergent industry.
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Extremophiles. 2000 Apr;4(2):91-8 PubMed
J Struct Biol. 2012 Feb;177(2):469-76 PubMed
Extremophiles. 2004 Dec;8(6):447-54 PubMed
Extremophiles. 2001 Apr;5(2):73-83 PubMed
Appl Microbiol Biotechnol. 2011 Apr;90(2):565-72 PubMed
J Ind Microbiol Biotechnol. 2012 Jul;39(7):965-75 PubMed
Appl Biochem Biotechnol. 2012 Mar;166(6):1442-53 PubMed
J Colloid Interface Sci. 2012 Aug 15;380(1):113-20 PubMed
Dermatitis. 2010 May-Jun;21(3):154-6 PubMed
Biosci Biotechnol Biochem. 2014;78(4):722-8 PubMed
J Food Sci. 2012 May;77(5):C506-11 PubMed
J Ind Microbiol Biotechnol. 2012 Apr;39(4):547-55 PubMed
J Microbiol Biotechnol. 2012 Mar;22(3):331-8 PubMed
Folia Microbiol (Praha). 2014 Sep;59(5):423-31 PubMed
J Microbiol. 2008 Jun;46(3):344-9 PubMed
Appl Biochem Biotechnol. 2013 Sep;171(2):382-95 PubMed
Appl Microbiol Biotechnol. 2008 May;79(2):165-78 PubMed
J Biosci Bioeng. 2012 May;113(5):568-74 PubMed
J Microbiol Biotechnol. 2008 Jan;18(1):160-6 PubMed
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4 PubMed
J Biosci Bioeng. 2011 Dec;112(6):551-7 PubMed
Prep Biochem Biotechnol. 2014;44(4):392-417 PubMed
Biosci Biotechnol Biochem. 2009 Jan;73(1):109-16 PubMed
Biomed Res Int. 2014;2014:345980 PubMed
Arch Microbiol. 2014 Jan;196(1):17-23 PubMed
Appl Microbiol Biotechnol. 2013 Sep;97(18):8121-8 PubMed
Crit Rev Microbiol. 2012 Nov;38(4):330-8 PubMed
Nucleic Acids Res. 2009 Jan;37(Database issue):D233-8 PubMed
Anal Biochem. 1976 May 7;72:248-54 PubMed
Appl Microbiol Biotechnol. 2012 Mar;93(5):1817-30 PubMed
Plant Physiol. 2007 Apr;143(4):1881-93 PubMed
Appl Microbiol Biotechnol. 2009 Mar;82(3):453-61 PubMed
