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A novel surfactant-, NaCl-, and protease-tolerant β-mannanase from Bacillus sp. HJ14

Zhang, Rui
Author Zhang, Rui Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan, Kunming, 650500, People's Republic of China Key Laboratory of Enzyme Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China
Song, Zhifeng
Author Song, Zhifeng College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China
Wu, Qian
Author Wu, Qian Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan, Kunming, 650500, People's Republic of China Key Laboratory of Enzyme Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China
Zhou, Junpei
Author Zhou, Junpei Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan, Kunming, 650500, People's Republic of China Key Laboratory of Enzyme Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China
Li, Junjun
Author Li, Junjun Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan, Kunming, 650500, People's Republic of China Key Laboratory of Enzyme Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China
Mu, Yuelin
Author Mu, Yuelin Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan, Kunming, 650500, People's Republic of China Key Laboratory of Enzyme Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China
Tang, Xianghua
Author Tang, Xianghua Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan, Kunming, 650500, People's Republic of China Key Laboratory of Enzyme Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China
Xu, Bo
Author Xu, Bo Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan, Kunming, 650500, People's Republic of China Key Laboratory of Enzyme Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China
Ding, Junmei
Author Ding, Junmei Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan, Kunming, 650500, People's Republic of China Key Laboratory of Enzyme Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China
Deng, Shucan
Author Deng, Shucan College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China
Huang, Zunxi
Author Huang, Zunxi Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China. huangzunxi@163.com College of Life Sciences, Yunnan Normal University, No.1 Yuhua District, Chenggong, Kunming, Yunnan, 650500, People's Republic of China. huangzunxi@163.com Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan, Kunming, 650500, People's Republic of China. huangzunxi@163.com Key Laboratory of Enzyme Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China. huangzunxi@163.com

Folia microbiologica. 2016 May ; 61 (3) : 233-42. [epub] 20151021

Folia Microbiol (Praha)
ISSN 1874-9356 | 0015-5632
Source

Language English Country United States Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid26489953

PubMed 26489953
DOI 10.1007/s12223-015-0430-y
PII: 10.1007/s12223-015-0430-y
Knihovny.cz E-resources

MeSH
Enzyme Activation drug effects MeSH
Bacillus drug effects genetics metabolism MeSH
beta-Mannosidase chemistry genetics isolation & purification metabolism MeSH
Sodium Chloride pharmacology MeSH
Detergents pharmacology MeSH
Endopeptidases metabolism MeSH
Gene Expression MeSH
Genome, Bacterial MeSH
Hydrolysis MeSH
Ions MeSH
Metals MeSH
Surface-Active Agents pharmacology MeSH
Recombinant Proteins MeSH
Amino Acid Sequence MeSH
Sequence Analysis, DNA MeSH
Enzyme Stability drug effects MeSH
Salt Tolerance * MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Names of Substances
beta-Mannosidase MeSH
Sodium Chloride MeSH
Detergents MeSH
Endopeptidases MeSH
Ions MeSH
Metals MeSH
Surface-Active Agents MeSH
Recombinant Proteins MeSH

A glycoside hydrolase family 5 β-mannanase-encoding gene was cloned from Bacillus sp. HJ14 isolated from saline soil in Heijing town. Coding sequence of mature protein (without the predicted signal peptide from M1 to A30) was successfully expressed in Escherichia coli BL21 (DE3). Purified recombinant mannanase (rMan5HJ14) exhibited optimal activity at pH 6.5 and 65 °C. The enzyme showed good salt tolerance, retaining more than 56 % β-mannanase activity at 3.0-30.0 % (w/v) NaCl and more than 94 % of the initial activity after incubation with 3.0-30.0 % (w/v) NaCl at 37 °C for 60 min. Almost no mannanase activity was lost after incubation of rMan5HJ14 with trypsin, proteinase K, and Alcalase at 37 °C for 60 min. Surfactants and chelating agents, namely SDS, CTAB, Tween 80, Triton X-100, EDTA, and sodium tripolyphosphate, showed little or no effect (retaining >82.4 % activity) on enzymatic activity. Liquid detergents, namely Tupperware, Walch, Bluemoon, Tide, and OMO, also showed little or no effect (retaining >72.4 % activity) on enzymatic activity at 0.5-2.0 % (v/v). The enzyme further presents a high proportion (11.97 %) of acidic amino acid residues (D and E), which may affect the SDS and NaCl tolerance of the enzyme. Together, the mannanase may be an alternative for potential use in liquid detergent industry.

College of Life Sciences Yunnan Normal University No 1 Yuhua District Chenggong Kunming Yunnan 650500 People's Republic of China

Engineering Research Center of Sustainable Development and Utilization of Biomass Energy Ministry of Education Yunnan Normal University Kunming 650500 People's Republic of China

Key Laboratory of Enzyme Engineering Yunnan Normal University Kunming 650500 People's Republic of China

Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment Yunnan Kunming 650500 People's Republic of China

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