Interactions of dendritic glycopolymer with erythrocytes, red blood cell ghosts and membrane enzymes
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26498371
DOI
10.1016/j.ijpharm.2015.10.046
PII: S0378-5173(15)30315-X
Knihovny.cz E-zdroje
- Klíčová slova
- Dendritic glycopolymer, Erythrocytes, Hemolysis, Nanoparticles,
- MeSH
- adenosintrifosfatasy metabolismus MeSH
- erytrocytární membrána účinky léků MeSH
- erytrocyty účinky léků MeSH
- fluorescenční spektrometrie MeSH
- iminy chemie farmakologie MeSH
- lidé MeSH
- maltosa chemie farmakologie MeSH
- nanočástice MeSH
- polyethyleny chemie farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adenosintrifosfatasy MeSH
- iminy MeSH
- maltosa MeSH
- poly(ethylene imine) MeSH Prohlížeč
- polyethyleny MeSH
Interactions between maltose functionalized hyperbranched poly(ethylene imine)s (95% maltose decoration denoted as Mal-PEI A; 33% maltose decoration denoted as Mal-PEI B) and red blood cells (RBCs) and between red blood cell membranes were investigated. We monitored the degree of hemolysis, the change in cell shape, the influence of polymers on the fluidity of the cell membrane and some cell membrane enzymes to determine their possible cytotoxic impact on them. To observe the extent of hemolysis, the RBCs were incubated with different concentrations of Mal-PEIs. The first significant lysis of RBCs was observed after 6h of incubation. Prolongation of the incubation time increased the number of ruptured cells. Moreover, we observed that Mal-PEI B was more hemolytic than Mal-PEI A in buffer solution. In contrast, an incubation of RBCs with Mal-PEIs in human plasma significantly decreased the hemolytic process and showed higher hemolytic property of Mal-PEI A compared to Mal-PEI B. Also several changes in the shape of the RBCs occurred after incubation with Mal-PEIs. Some of the erythrocytes shrank (echinocytes), but their morphology generally remained unchanged during the incubation. As shown by fluorescence experiments, both polymers induced the increase of fluidity of RBCs membranes. In summary, both types of hyperbranched poly(ethylene imine)s were practically non-hemolytic even at high polymer concentrations. Mal-PEI B was slightly more noxious than the Mal-PEI A in a buffer solution, while in blood plasma, the situation was opposite. Decrease of Na+/K+ ATPase and total ATPase enzymes activity was related with molecule size and number of maltose groups on the surface of molecule. The low hemolytic properties only observed at higher concentration (100μM and 400μM) indicated that Mal-PEIs are promising macromolecules in the area of drug delivery systems.
Department of Biology Jan Evangelista Purkinje University Usti nad Labem Czech Republic
Leibniz Institut für Polymerforschung Dresden e 5 Hohe Straße 6 01069 Dresden Germany
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