In this study, we have developed a combined approach to accelerate the proliferation of mesenchymal stem cells (MSCs) in vitro, using a new nanofibrous scaffold made by needleless electrospinning from a mixture of poly-ε-caprolactone and magnetic particles. The biological characteristics of porcine MSCs were investigated while cultured in vitro on composite scaffold enriched with magnetic nanoparticles. Our data indicate that due to the synergic effect of the poly-ε-caprolactone nanofibers and magnetic particles, cellular adhesion and proliferation of MSCs is enhanced and osteogenic differentiation is supported. The cellular and physical attributes make this new scaffold very promising for the acceleration of efficient MSC proliferation and regeneration of hard tissues.

Department of Magnetic Nanosystems Institute of Physics Academy of Sciences of the Czech Republic Prague Czech Republic

Faculty of Veterinary Medicine University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic

Laboratory of Tissue Engineering Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic ; Faculty of Biomedical Engineering Czech Technical University Prague Kladno Czech Republic ; University Center for Energy Efficient Buildings Czech Technical University Prague Bustehrad Czech Republic

Laboratory of Tissue Engineering Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic ; Institute of Biophysics 2nd Faculty of Medicine Charles University Prague Prague Czech Republic

Laboratory of Tissue Engineering Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic ; Institute of Biophysics 2nd Faculty of Medicine Charles University Prague Prague Czech Republic ; Faculty of Biomedical Engineering Czech Technical University Prague Kladno Czech Republic

University Center for Energy Efficient Buildings Czech Technical University Prague Bustehrad Czech Republic ; Department of Nonwoven Textiles Faculty of Textile Engineering Technical University of Liberec Liberec Czech Republic

Zobrazit více v PubMed

Scherer F, Anton M, Schillinger U, et al. Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo. Gene Ther. 2002;9(2):102–109. PubMed

Mykhaylyk O, Antequera YS, Vlaskou D, Plank C. Generation of magnetic nonviral gene transfer agents and magnetofection in vitro. Nat Protoc. 2007;2(10):2391–2411. PubMed

Shinkai M, Yanase M, Honda H, Wakabayashi T, Yoshida J, Kobayashi T. Intracellular hyperthermia for cancer using magnetite cationic liposomes: in vitro study. Jpn J Cancer Res. 1996;87(11):1179–1183. PubMed PMC

Ito A, Hibino E, Shimizu K, et al. Magnetic force-based mesenchymal stem cell expansion using antibody-conjugated magnetoliposomes. J Biomed Mater Res B Appl Biomater. 2005;75(2):320–327. PubMed

Vadala ML, Zalich MA, Fulks DB, St Pierre TG, Dailey JP, Riffle JS. Cobalt–silica magnetic nanoparticles with functional surfaces. J Magn Magn Mater. 2005;293(1):162–170.

Müller R, Steinmetz H, Hiergeist R, Gawalek W. Magnetic particles for medical applications by glass crystallisation. J Magn Magn Mater. 2004;272–276(Pt 2):1539–1541.

Häfeli UO, Pauer GJ. In vitro and in vivo toxicity of magnetic microspheres. J Magn Magn Mater. 1999;194(1–3):76–82.

Barry SE. Challenges in the development of magnetic particles for therapeutic applications. Int J Hyperthermia. 2008;24(6):451–466. PubMed

Deng ZL, Sharff KA, Tang N, et al. Regulation of osteogenic differentiation during skeletal development. Front Biosci. 2008;13:2001–2021. PubMed

Friedenstein AJ, Petrakova KV, Kurolesova AI, Frolova GP. Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation. 1968;6(2):230–247. PubMed

Luu HH, Song WX, Luo X, et al. Distinct roles of bone morphogenetic proteins in osteogenic differentiation of mesenchymal stem cells. J Orthop Res. 2007;25(5):665–677. PubMed

D’Ippolito G, Diabira S, Howard GA, Roos BA, Schiller PC. Low oxygen tension inhibits osteogenic differentiation and enhances stemness of human MIAMI cells. Bone. 2006;39(3):513–522. PubMed

Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143–147. PubMed

Sill TJ, von Recum HA. Electrospinning: applications in drug delivery and tissue engineering. Biomaterials. 2008;29(13):1989–2006. PubMed

Lukáš D, Sarkar A, Martinová L, et al. Physical principles of electro-spinning. Text Prog. 2009;41(2):59–140.

Puppi D, Chiellini F, Piras AM, Chiellini E. Polymeric materials for bone and cartilage repair. Prog Polym Sci. 2010;35(4):403–440.

Rampichova M, Chvojka J, Buzgo M, et al. Elastic three-dimensional poly (epsilon-caprolactone) nanofibre scaffold enhances migration, proliferation and osteogenic differentiation of mesenchymal stem cells. Cell Prolif. 2013;46(1):23–37. PubMed PMC

Woodruff MA, Hutmacher DW. The return of a forgotten polymer – Polycaprolactone in the 21st century. Prog Polym Sci. 2010;35(10):1217–1256.

Roca AG, Niznansky D, Poltierova-Vejpravova J, et al. Magnetite nanoparticles with no surface spin canting. J Appl Phys. 2009;105(11):7.

Plencner M, East B, Tonar Z, et al. Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-epsilon-caprolactone nanofibers and growth factors for prevention of incisional hernia formation. Int J Nanomed. 2014;9:3263–3277. PubMed PMC

Mickova A, Buzgo M, Benada O, et al. Core/shell nanofibers with embedded liposomes as a drug delivery system. Biomacromolecules. 2012;13(4):952–962. PubMed

Prosecká E, Rampichová M, Litvinec A, et al. Collagen/hydroxyapatite scaffold enriched with polycaprolactone nanofibers, thrombocyte-rich solution and mesenchymal stem cells promotes regeneration in large bone defect in vivo. J Biomed Mater Res A. 2015;103(2):671–682. PubMed

Zeng XB, Hu H, Xie LQ, et al. Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation. Int J Nanomed. 2012;7:3365–3378. PubMed PMC

Singh RK, Patel KD, Lee JH, et al. Potential of magnetic nanofiber scaffolds with mechanical and biological properties applicable for bone regeneration. PLoS One. 2014;9(4):e91584. PubMed PMC

Agarwal K, Prasad M, Sharma RB, Setua DK. Studies on microstructural and thermophysical properties of polymer nanocomposite based on polyphenylene oxide and ferrimagnetic iron oxide. Polym Test. 2011;30(1):155–160.

Sadeghi L, Tanwir F, Yousefi Babadi V. In vitro toxicity of iron oxide nanoparticle: oxidative damages on Hep G2 cells. Exp Toxicol Pathol. 2015;67(2):197–203. PubMed

Kannarkat JT, Battogtokh J, Philip J, Wilson OC, Mehl PM. Embedding of magnetic nanoparticles in polycaprolactone nanofiber scaffolds to facilitate bone healing and regeneration. J Appl Phys. 2010;107(9):09. B307.

Cai Q, Shi Y, Shan D, et al. Osteogenic differentiation of MC3T3-E1 cells on poly(l-lactide)/Fe3O4 nanofibers with static magnetic field exposure. Mater Sci Eng C Mater Biol Appl. 2015;55:166–173. PubMed

Wei Y, Zhang X, Song Y, et al. Magnetic biodegradable Fe3O4/CS/PVA nanofibrous membranes for bone regeneration. Biomed Mater. 2011;6(5):055008. PubMed

Hu H, Jiang W, Lan F, et al. Synergic effect of magnetic nanoparticles on the electrospun aligned superparamagnetic nanofibers as a potential tissue engineering scaffold. RSC Adv. 2013;3(3):879–886.

Hou R, Zhang G, Du G, et al. Magnetic nanohydroxyapatite/PVA composite hydrogels for promoted osteoblast adhesion and proliferation. Colloids Surf B Biointerfaces. 2013;103:318–325. PubMed

Lai K, Jiang W, Tang JZ, et al. Superparamagnetic nanocomposite scaffolds for promoting bone cell proliferation and defect reparation without a magnetic field. RSC Adv. 2012;2(33):13007–13017.

Gloria A, Russo T, D’Amora U, et al. Magnetic poly(epsilon-caprolactone)/iron-doped hydroxyapatite nanocomposite substrates for advanced bone tissue engineering. J R Soc Interface. 2013;10(80):20120833. PubMed PMC

Wang M, Castro NJ, Li J, Keidar M, Zhang LG. Greater osteoblast and mesenchymal stem cell adhesion and proliferation on titanium with hydro-thermally treated nanocrystalline hydroxyapatite/magnetically treated carbon nanotubes. J Nanosci Nanotechnol. 2012;12(10):7692–7702. PubMed

Tran N, Webster TJ. Increased osteoblast functions in the presence of hydroxyapatite-coated iron oxide nanoparticles. Acta Biomater. 2011;7(3):1298–1306. PubMed

Riegler J, Liew A, Hynes SO, et al. Superparamagnetic iron oxide nanoparticle targeting of MSCs in vascular injury. Biomaterials. 2013;34(8):1987–1994. PubMed

Ju S, Teng GJ, Lu H, et al. In vivo differentiation of magnetically labeled mesenchymal stem cells into hepatocytes for cell therapy to repair damaged liver. Invest Radiol. 2010;45(10):625–633. PubMed

Hughes S, El Haj AJ, Dobson J. Magnetic micro- and nanoparticle mediated activation of mechanosensitive ion channels. Med Eng Phys. 2005;27(9):754–762. PubMed

Kirkham GR, Elliot KJ, Keramane A, et al. Hyperpolarization of human mesenchymal stem cells in response to magnetic force. IEEE Trans Nanobiosci. 2010;9(1):71–74. PubMed

Cartmell SH, Hughes S, Dobson J, El Haj A. Preliminary analysis of magnetic particle techniques for activating mechanotransduction in bone cells; Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, 2002; New York, NY: IEEE; 2002.

Lang SB. Pyroelectric effect in bone and tendon. Nature. 1966;212(5063):704–705.

Huang DM, Hsiao JK, Chen YC, et al. The promotion of human mesenchymal stem cell proliferation by superparamagnetic iron oxide nanoparticles. Biomaterials. 2009;30(22):3645–3651. PubMed

Nanomaterials in Skin Regeneration and Rejuvenation