Effect of metal ions on autofluorescence of the dry rot fungus Serpula lacrymans grown on spruce wood
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26873389
DOI
10.1007/s12223-015-0415-x
PII: 10.1007/s12223-015-0415-x
Knihovny.cz E-zdroje
- MeSH
- Basidiomycota chemie růst a vývoj metabolismus účinky záření MeSH
- buněčná stěna chemie metabolismus účinky záření MeSH
- dřevo mikrobiologie MeSH
- fluorescence MeSH
- kovy metabolismus MeSH
- mycelium chemie růst a vývoj metabolismus účinky záření MeSH
- smrk mikrobiologie MeSH
- ultrafialové záření MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kovy MeSH
This work describes autofluorescence of the mycelium of the dry rot fungus Serpula lacrymans grown on spruce wood blocks impregnated with various metals. Live mycelium, as opposed to dead mycelium, exhibited yellow autofluorescence upon blue excitation, blue fluorescence with ultraviolet (UV) excitation, orange-red and light-blue fluorescence with violet excitation, and red fluorescence with green excitation. Distinctive autofluorescence was observed in the fungal cell wall and in granula localized in the cytoplasm. In dead mycelium, the intensity of autofluorescence decreased and the signal was diffused throughout the cytoplasm. Metal treatment affected both the color and intensity of autofluorescence and also the morphology of the mycelium. The strongest yellow signal was observed with blue excitation in Cd-treated samples, in conjunction with increased branching and the formation of mycelial loops and protrusions. For the first time, we describe pink autofluorescence that was observed in Mn-, Zn-, and Cu-treated samples with UV, violet or. blue excitation. The lowest signals were obtained in Cu- and Fe-treated samples. Chitin, an important part of the fungal cell wall exhibited intensive primary fluorescence with UV, violet, blue, and green excitation.
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