Psocids are important stored product pests found worldwide that can be spread through grain trade. Most stored-product psocids, including eggs, nymphs, and adults, are very small (~1 mm) and difficult to identify morphologically. Here, we collected 10 economically important stored-product Liposcelis spp. psocids (L. bostrychophila, L. entomophila, L. decolor, L. paeta, L. brunnea, L. corrodens, L. mendax, L. rufa, L. pearmani, and L. tricolor) from 35 geographical locations in 5 countries (China, Czech Republic, Denmark, Germany, and the United States). The ITS2 rDNA gene was extracted and sequenced. The interspecific genetic distance of the stored-product psocids was significantly higher than the intraspecific genetic distance according to the barcoding gap analysis. Ten pairs of species-specific primers based on the ITS2 rDNA were developed for psocid identification. The sensitivity estimation indicated that the species-specific primers could correctly amplify the target ITS2 gene and successfully identify psocids at 1.0 ng/mL. Additionally, these species-specific primers could quantify specificity and identify 10 stored-product psocids; this approach could also be used to accurately identify other stored-product psocids. This work provides a practical approach for the precise examination of 10 stored-product psocid species and also contributes to the development of an identification method using ITS2 rDNA.

Academy of State Administration of Grain Beijing 100037 China

College of Life Science China Jiliang University Hangzhou 310018 China

Crop Research Institute Drnovská 507 161 06 Prague 6 Czech Republic

Department of Entomology and Plant Pathology 127 Noble Research Center Oklahoma State University Stillwater OK 74078 USA

Department of Entomology College of Plant Protection China Agricultural University Beijing 100193 China

Institute of Plant Quarantine Research Chinese Academy of Inspection and Quarantine Beijing 100176 China

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Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA