Physiology of Ca(2+) signalling in stem cells of different origins and differentiation stages
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
26905828
DOI
10.1016/j.ceca.2016.02.001
PII: S0143-4160(16)30006-9
Knihovny.cz E-resources
- Keywords
- Adipose tissue derived mesenchymal stromal stem cells, Bone marrow mesenchymal stromal cells, Ca(2+) channels, Ca(2+) signalling, Cell differentiation, Cell therapy, Glutamate, Human embryonic stem cells, Human induced pluripotent stem cells, Inositol trisphosphate, Intracellular [Ca(2+)](i) stores, Neural precursors, Neurodegeneration, Oxytocin, Purinergic receptors, Ryanodine receptors, Spontaneous Ca(2+) oscillations, Stem cells, Transplantation, Vasopressin,
- MeSH
- Cell Differentiation * MeSH
- Stem Cells cytology metabolism MeSH
- Humans MeSH
- Calcium metabolism MeSH
- Calcium Signaling * MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Calcium MeSH
Stem cells (SCs) of different origins have brought hope as potential tools for the treatment of neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and Amyotrophic Lateral Sclerosis. Calcium signalling plays a key role in SC differentiation and proliferation, and dysregulation of Ca(2+) homeostasis may instigate pathological scenarios. Currently, the role of ion channels and receptors in SCs is not fully understood. In the recent years, we found that (i) the pre-differentiation of human embryonic SCs (hESCs) led to the activation of Ca(2+) signalling cascades and enhanced the functional activities of these cells, (ii) the Ca(2+) homeostasis and the physiological properties of hESC-derived neural precursors (NPs) changed during long term propagation in vitro, (iii) differentiation of NPs derived from human induced pluripotent SCs affects the expression of ion channels and receptors, (iv) these neuronal precursors exhibited spontaneous activity, indicating that their electrophysiological and Ca(2+) handling properties are similar to those of mature neurones, and (v) in mesenchymal SCs isolated from the adipose tissue and bone marrow of rats the expression profile of ion channels and receptors depends not only on the differentiation conditions but also on the source from which the cells were isolated, indicating that the fate and functional properties of the differentiated cells are driven by intrinsic mechanisms. Together, identification and assignment of a unique ion channel and a Ca(2+) handling footprint for each cell type would be necessary to qualify them as physiologically suitable for medical research, drug screening, and cell therapy.
References provided by Crossref.org
Mesenchymal Stem Cells in Treatment of Spinal Cord Injury and Amyotrophic Lateral Sclerosis
Neuroprotective Potential of Cell-Based Therapies in ALS: From Bench to Bedside
