Exploring complex pheromone biosynthetic processes in the bumblebee male labial gland by RNA sequencing
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26945888
DOI
10.1111/imb.12221
Knihovny.cz E-zdroje
- Klíčová slova
- Bombus terrestris, RNA-seq, apoptosis, labial gland, marking pheromone biosynthesis, transcriptome,
- MeSH
- aldehydoxidoreduktasy metabolismus MeSH
- apoptóza MeSH
- desaturasy mastných kyselin metabolismus MeSH
- feromony biosyntéza MeSH
- hydrolýza MeSH
- mastné kyseliny biosyntéza MeSH
- metabolismus lipidů * MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce MeSH
- sekvence aminokyselin MeSH
- sekvenční analýza RNA MeSH
- terpeny metabolismus MeSH
- transkriptom MeSH
- tukové těleso metabolismus MeSH
- včely metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aldehydoxidoreduktasy MeSH
- desaturasy mastných kyselin MeSH
- fatty acid reductase MeSH Prohlížeč
- feromony MeSH
- mastné kyseliny MeSH
- terpeny MeSH
Male marking pheromones (MPs) are used by the majority of bumblebee species (Hymenoptera: Apidae), including a commercially important greenhouse pollinator, the buff-tailed bumblebee (Bombus terrestris), to attract conspecific females. MP biosynthetic processes in the cephalic part of the bumblebee male labial gland (LG) are of extraordinary complexity, involving enzymes of fatty acid and isoprenoid biosynthesis, which jointly produce more than 50 compounds. We employed a differential transcriptomic approach to identify candidate genes involved in MP biosynthesis by sequencing Bombus terrestris LG and fat body (FB) transcriptomes. We identified 12 454 abundantly expressed gene products (reads per kilobase of exon model per million mapped reads value > 1) that had significant hits in the GenBank nonredundant database. Of these, 876 were upregulated in the LG (> 4-fold difference). We identified more than 140 candidate genes potentially involved in MP biosynthesis, including esterases, fatty acid reductases, lipases, enzymes involved in limited fatty acid chain shortening, neuropeptide receptors and enzymes involved in biosynthesis of triacylglycerols, isoprenoids and fatty acids. For selected candidates, we confirmed their abundant expression in LG using quantitative real-time reverse transcription-PCR (qRT-PCR). Our study shows that the Bombus terrestris LG transcriptome reflects both fatty acid and isoprenoid MP biosynthetic processes and identifies rational gene targets for future studies to disentangle the molecular basis of MP biosynthesis. Additionally, LG and FB transcriptomes enrich the available transcriptomic resources for Bombus terrestris.
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