A water-soluble polysaccharide JS-MP-1 was isolated from Korean mulberry fruits Oddi (Morus alba L.). Sugar linkage analysis and NMR data confirmed that it is a rhamnogalacturonan type I (RG I) polymer carrying arabinan and arabinogalactan (AG II) side chains. JS-MP-1 reduced dose-dependently the viability of 3T3-L1 pre-adipocyte cells, significantly stimulated the cleavage of caspases 9 and 3 and poly (ADP-ribose) polymerase (PARP) and decreased the ratio of Bcl-2 to Bax expression level that led to mitochondrial dysfunction and apoptosis in pre-adipocyte cells. The apoptotic death was mediated by stimulation of MAPKs (ERK and p38) signalling pathway. These results suggest that JS-MP-1 is able to reduce the number of fat cells and the mass of adipose tissue via inhibition of pre-adipocyte proliferation and thus JS-MP-1 itself or a crude aqueous Oddi extract containing this polysaccharide can be used as functional ingredient of health-beneficial food supplements for the treatment or prevention of obesity disorders.

Department of Biotechnology The Catholic University of Korea Bucheon Gyeonggi do 420 743 Republic of Korea Republic of Korea

Department of Carbohydrates and Cereals University of Chemical Technology Prague Technická 5 166 28 Prague 6 Czech Republic

Institute of Chemistry Centre for Glycomics Slovak Academy of Sciences Dúbravská cesta 9 845 38 Bratislava Slovakia

Institute of Organic Chemistry and Biochemistry AS CR Flemingovo sq 2 166 28 Prague 6 Czech Republic

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