- MeSH
- deprese farmakoterapie MeSH
- klinické zkoušky jako téma MeSH
- kognice MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- Morus * chemie MeSH
- myši MeSH
- nervový systém * účinky léků MeSH
- neurodegenerativní nemoci farmakoterapie prevence a kontrola MeSH
- neuroprotektivní látky * farmakologie terapeutické užití MeSH
- preklinické hodnocení léčiv MeSH
- psychický stres farmakoterapie MeSH
- rostlinné extrakty farmakologie terapeutické užití MeSH
- stárnutí účinky léků MeSH
- tradiční orientální medicína MeSH
- úzkost farmakoterapie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
Chromatographic separation of root extracts of Morus alba and M. nigra led to the identification of the 2-arylbenzofurans moracin C (1), mulberrofuran Y (2), and mulberrofuran H (3), and the prenylated flavonoids kuwanon E (4), kuwanon C (5), sanggenon H (6), cudraflavone B (7), and morusinol (8), and the Diels-Alder adducts soroceal (9), and sanggenon E (10). The cytotoxicity and their antiphlogistic activity, determined as the attenuation of the secretion of TNF-α and IL-1β and the inhibition of NF-κB nuclear translocation in LPS-stimulated macrophages, were evaluated for compounds 1-10.
- MeSH
- antiflogistika farmakologie MeSH
- benzofurany chemie izolace a purifikace farmakologie MeSH
- benzopyrany chemie izolace a purifikace farmakologie MeSH
- flavonoidy chemie izolace a purifikace farmakologie MeSH
- kořeny rostlin chemie MeSH
- kůra rostlin chemie MeSH
- lidé MeSH
- lipopolysacharidy farmakologie MeSH
- makrofágy účinky léků MeSH
- molekulární struktura MeSH
- Morus chemie MeSH
- myši MeSH
- NF-kappa B účinky léků MeSH
- prenylace MeSH
- TNF-alfa farmakologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Dry matter, organic acids, ascorbic acid, minerals (nitrogen, phosphorus, potassium, calcium, magnesium, sodium) and polyphenolic profile of a number of non-traditional fruit species and their genotypes, namely blue honeysuckle (Lonicera spp.), Saskatoon berry (Amelanchier alnifolia), black mulberry (Morus nigra), Tomentosa cherry (Prunus tomentosa Thunb.) and jostaberry (Ribes nigrum x Grossularia uva-crispa) were investigated. The results showed that Lonicera genotypes displayed high levels of ascorbic acid and they were rich in minerals, with the cultivar ‘Amfora’ achieving the leading position in nitrogen, phosphorus and potassium content among all lesser known fruit species. Amelanchier cultivars represented a valuable source of ascorbic acid and calcium, ‘Tišňovský’ and ‘Smoky’ together with Morus nigra ‘Jugoslavska’ accumulated the highest level of examined polyphenolic compounds. Regular consumption of studied less common fruit species can bring health benefits so they can represent a high potential value for fruit growers and in addition they can be utilised as functional foods.
- MeSH
- genotyp MeSH
- kyseliny karboxylové analýza izolace a purifikace MeSH
- kyseliny nekarboxylové analýza izolace a purifikace MeSH
- Lonicera chemie MeSH
- minerály analýza izolace a purifikace MeSH
- Morus chemie MeSH
- nutriční hodnota * MeSH
- ovoce chemie MeSH
- polyfenoly analýza izolace a purifikace MeSH
- Ribes chemie MeSH
- Rosaceae chemie MeSH
- rostlinné extrakty analýza izolace a purifikace MeSH
- slivoň chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
Cudraflavone B (1) is a prenylated flavonoid found in large amounts in the roots of Morus alba, a plant used as a herbal remedy for its reputed anti-inflammatory properties. The present study shows that this compound causes a significant inhibition of inflammatory mediators in selected in vitro models. Thus, 1 was identified as a potent inhibitor of tumor necrosis factor α (TNFα) gene expression and secretion by blocking the translocation of nuclear factor κB (NF-κB) from the cytoplasm to the nucleus in macrophages derived from a THP-1 human monocyte cell line. The NF-κB activity reduction resulted in the inhibition of cyclooxygenase 2 (COX-2) gene expression. Compound 1 acts as a COX-2 and COX-1 inhibitor with higher selectivity toward COX-2 than indomethacin. Pretreatment of cells by 1 shifted the peak in an regulatory gene zinc-finger protein 36 (ZFP36) expression assay. This natural product has noticeable anti-inflammatory properties, suggesting that 1 potentially could be used for development as a nonsteroidal anti-inflammatory drug lead.
- MeSH
- aktiny účinky léků MeSH
- antiflogistika nesteroidní chemie izolace a purifikace farmakologie MeSH
- cyklooxygenasa 1 účinky léků MeSH
- flavonoidy chemie izolace a purifikace farmakologie MeSH
- inhibitory cyklooxygenasy 2 chemie izolace a purifikace farmakologie MeSH
- kořeny rostlin chemie MeSH
- lidé MeSH
- makrofágy účinky léků MeSH
- molekulární struktura MeSH
- Morus chemie MeSH
- NF-kappa B antagonisté a inhibitory MeSH
- TNF-alfa antagonisté a inhibitory genetika MeSH
- tristetraprolin účinky léků genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Nine geranylated flavanones isolated from the fruits of Paulownia tomentosa (4-12) and two from the roots of Morus alba (13 and 14) were examined for cytotoxicity to selected human cancer cell lines and normal human fibroblasts. Cytotoxicity was determined in vitro using a calcein AM cytotoxicity assay. Cytotoxicity for the THP-1 monocytic leukemia cell line was tested using erythrosin B cell staining. The geranylated compounds tested were compared with the known simple flavanone standards taxifolin (1), naringenin (2), and hesperetin (3) and with the standard anticancer drugs olomoucine II, diaziquone, and oxaliplatin and the antineoplastic compound camptothecin, and showed different levels of cytotoxicity. The effects of structural changes on cytotoxic activity, including geranyl substitution of the flavanone skeleton and the oxidation pattern of ring B of the flavanones, are discussed.
- MeSH
- antitumorózní látky fytogenní chemie izolace a purifikace farmakologie MeSH
- apoptóza účinky léků MeSH
- fibroblasty účinky léků MeSH
- flavanony chemie izolace a purifikace farmakologie MeSH
- kořeny rostlin chemie MeSH
- léčivé rostliny chemie MeSH
- léky antitumorózní - screeningové testy MeSH
- lidé MeSH
- Magnoliopsida chemie MeSH
- molekulární struktura MeSH
- Morus chemie MeSH
- nukleární magnetická rezonance biomolekulární MeSH
- ovoce chemie MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Turecko MeSH
